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Method for preparing arctigenin

A technology of aglycone and burdock, which is applied in the field of preparation of burdock extract, can solve the problems of slow reaction time and low hydrolysis efficiency, and achieve the effects of increasing content, high conversion efficiency, and improving extraction rate

Inactive Publication Date: 2016-06-01
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first method uses the hydrolytic enzyme of the bovine bladder itself for hydrolysis, and the second method directly hydrolyzes under acidic conditions. The reaction time between the two is slow and the hydrolysis efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of bovis aglycone includes the following steps: (1) activation of bacterial species: take out the freeze-dried powder of bacterial species that is stored in a vacuum drying tube and stored at low temperature, restore it to room temperature, and transfer it to an ultra-clean workbench to stain it with Cotton with 75% alcohol, wipe the outer tube, heat the tip of the outer tube on the flame, drop a few drops of sterile water on the heating place to break the outer tube, take out the heat insulation and evenly spread it on the PDA plate, separate and culture for 2 days, and culture The temperature is 30℃;

[0028] (2) Preparation of seed bacteria liquid: inoculate the activated strain on the PDA plate, place it in a constant temperature incubator and cultivate it at 30°C for 6 days, add sterile water to the plate and shake, wash off the spores on the surface of the agar, filter, and obtain Spore suspension, calculated spore concentration, and formulate...

Embodiment 2

[0033] A preparation method of bovis aglycone includes the following steps: (1) activation of bacterial species: take out the freeze-dried powder of bacterial species that is stored in a vacuum drying tube and stored at low temperature, restore it to room temperature, and transfer it to an ultra-clean workbench to stain it with Cotton with 75% alcohol, wipe the outer tube, heat the tip of the outer tube on the flame, drop a few drops of sterile water on the heating place to break the outer tube, take out the heat insulation and spread it evenly on the PDA plate, separate and culture for 1 day, and culture The temperature is 28℃;

[0034] (2) Preparation of seed bacteria liquid: inoculate the activated strain on a PDA plate, place it in a constant temperature incubator and cultivate it at 28°C for 5 days, add sterile water to the plate and shake it, wash off the spores on the surface of the agar, filter, and obtain Spore suspension, calculated spore concentration, and formulate...

Embodiment 3

[0039] A preparation method of bovis aglycone includes the following steps: (1) activation of bacterial species: take out the freeze-dried powder of bacterial species that is stored in a vacuum drying tube and stored at low temperature, restore it to room temperature, and transfer it to an ultra-clean workbench to stain it with Cotton with 75% alcohol, wipe the outer tube, heat the tip of the outer tube on the flame, drop a few drops of sterile water on the heating place to break the outer tube, take out the heat insulation and evenly spread it on the PDA plate, separate and culture for 1.5 days, The culture temperature is 29°C;

[0040] (2) Preparation of seed bacteria liquid: inoculate the activated strain on the PDA plate, place it in a constant temperature incubator and cultivate it at 29°C for 5.5 days, add sterile water to the plate and shake, wash off the spores on the surface of the agar, filter, Obtain the spore suspension, calculate the spore concentration, and make ...

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PUM

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Abstract

The invention discloses a method for preparing arctigenin. The method comprises the following steps of activating strains, preparing seed microbial liquid, fermenting fructus arctii powder through trichoderma reesei and aspergillus awamori, conducting ultrasonic-wave-assisted extraction to obtain fructus arctii crude extract and conducting silica gel column chromatography purification. According to the method, the fructus arctii powder serves as the raw material to prepare the fructus arctii extract through a biological fermentation technology, the fructus arctii powder is processed through fermentation of beta-glucosidase and a cellulase high-producing strain, the glucosidic bond of burdock glycoside is ruptured to be converted into the arctigenin under the in-vitro controllable condition, the content of arctigenin in fructus arctii is increased, a simple and effective extraction method is adopted, and high-purity arctigenin can be extracted.

Description

technical field [0001] The present invention relates to a preparation method of bovis extract, in particular to a preparation method of bovis aglycone. Background technique [0002] Arctium aglycone, chemical name: (3R,4R)-[(3,4-Dimethoxyphenyl)methy]dihydro-3-[(4-hydroxy-3-methoxyphenyl)methyl]-2(3H)-furanone, molecular formula: C21H24O6, molecular weight: 372.41. Recently, it has been reported that arctigenin shows strong pharmacological activities in anti-inflammatory, anti-viral, anti-tumor, immune regulation, etc.; at the same time, it can be used as a new type of heat shock response inhibitory regulator, which can be used to treat cancer caused by hyperthermia. Treatment is helpful. In addition, it also has the effect of anti-senile dementia and the effect of inhibiting K+ contracture. Arctium aglycone is a lignan compound, mainly derived from Arctium lappaL, the fruit of Arctium lappaL. Arctium seeds mainly contain arctiin and arctigenin. It has been verified tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P17/04C12N1/14C12R1/885C12R1/665
CPCC12P39/00C12N1/14C12P17/04
Inventor 蒋承建
Owner GUANGXI UNIV
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