Ageratina adenophora organic fertilizer solid microbial inoculant and preparation method thereof, and application of microbial inoculant in organic fertilizer production

A technology of E. japonica and solid inoculum, which is applied in the preparation of organic fertilizers, organic fertilizers, biochemical equipment and methods, etc., can solve the problem of low composting temperature, unsuitable for harmless treatment and resource utilization of E. japonica It is difficult to effectively kill the problems of vegetative reproductive organs and seeds of E. chinensis, and achieve the effect of large quantity, labor saving and large organism.

Inactive Publication Date: 2016-06-01
SOUTHWEST UNIVERSITY +1
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Problems solved by technology

However, the roots, stems and seeds of Eupatorium adenophorum can be regenerated and reproduced, and the method of returning crop straw to the field cannot be directly returned to the field for utilization
In addition, Eupatorium adenophorum also has microbial toxicity. During the natural composting process, the composting bio

Method used

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  • Ageratina adenophora organic fertilizer solid microbial inoculant and preparation method thereof, and application of microbial inoculant in organic fertilizer production

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Effect test

Embodiment 1

[0024] The preparation of embodiment 1 Eupatorium adenophorum organic fertilizer solid bacterial agent

[0025] 1. Preparation of the third-grade strains of Pseudomonas fluorescens in Eupatorium adenophorum organic fertilizer solid bacterial agent:

[0026] The first-class strain bacteria liquid: prepare solid medium (take 0.3 grams of beef extract, 1.0 grams of cornstarch, 2.0 grams of Eupatorium adenophorum powder that has been air-dried and ground through a 0.1 mm sieve, 0.5 grams of sodium chloride, 1.5 grams of agar, water 1000 ml, pH value 7.0-7.2), about 5 ml is placed in a 20 ml test tube, sterilized at 121 °C and 1.5 atmospheric pressure for 30 min, placed on a slope, and inoculated with Pseudomonas fluorescens after cooling , 35°C for 4 days in the dark. Bacterial colonies on the surface of the culture medium were washed with sterile water and diluted to 104 bacteria / ml for subsequent use;

[0027] Secondary strain bacteria liquid: prepare liquid culture medium (ta...

Embodiment 2

[0037] The investigation of embodiment 2 Eupatorium adenophorum organic fertilizer

[0038] In the Plant Nutrition and Microbiology Laboratory of Southwest University, the treatment bacteria were prepared according to the preparation method of the bacteria, packaged and transported to the place where Eupatoria adenophorum needs to be treated.

[0039] Eupatorium adenophorum was treated in Niulang Village, Xixi Township, Xichang City, Liangshan Prefecture, Sichuan Province (altitude 1800m). Harvest Eupatorium adenophorum in June 2014 and divide it into three groups; choose a flat plot, stack the three groups of fresh Eupatorium adenophorum parallel to the ground, each pile is 30-50cm thick, and sprinkle Eupatorium adenophorum separately Organic fertilizer solid bacterial agents A, B, C groups, urea and gypsum, trampled tightly, piled up to a height of 1-1.5m, covered with agricultural plastic film, 1000-1500kg per pile; said Eupatorium adenophorum and Eupatorium adenophorum Th...

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Abstract

The invention provides an Ageratina adenophora organic fertilizer solid microbial inoculant which is formed by mixing a tertiary strain of Pseudomonas fluorescens and a tertiary strain of Cellulomonas. The Ageratina adenophora whole plant is directly treated in situ in the open air; and thus, the method is economical and applicable, has the advantages of low cost, labor saving and wide application range, and is simple to operate and easy to popularize, thereby providing a new idea for solving the problem of Ageratina adenophora disasters.

Description

technical field [0001] The invention relates to a biologically fermented organic fertilizer, and more specifically relates to a solid bacterial agent of Eupatorium adenophorum organic fertilizer, a preparation method and an application in the production of organic fertilizer. technical background [0002] Eupatorium adenophorum Sprengel is a perennial herbaceous plant of the genus Eupatorium Linnaeus in the family Asteraceae. It is native to Mexico, Costa Rica, Uruguay, Jamaica, and Cuba in Central America. It was introduced to Europe as an ornamental plant, and then Introduced to Australia and Asia, it has been widely distributed in more than 30 tropical and subtropical countries and regions. After it invaded China in the 1940s and 1950s, it quickly evolved into the main invasive alien plant in Southwest China, causing serious damage to the local natural and agricultural ecosystems. In the past 10 years, its invasion trend is obvious, and it has spread from the original di...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05F17/00C12R1/39C12R1/01
CPCC05D3/00C05F17/00C05G3/00C12N1/20C05C9/00C05F11/00C05F11/08Y02W30/40
Inventor 袁玲黄建国杜如万徐明康吴叶宽王勇杨水平
Owner SOUTHWEST UNIVERSITY
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