Porcine reproductive and respiratory syndrome antibody detecting card and preparation method thereof

Abstract

The invention discloses a porcine reproductive and respiratory syndrome antibody detecting card and a preparation method thereof. The porcine reproductive and respiratory syndrome antibody detecting card comprises a detecting card body assembled in a shell, wherein the detecting card body comprises a bottom plate; a sample pad, a label pad, a nitrocellulose film and absorbent paper are sequentially arranged on the bottom plate; the nitrocellulose film is coated with porcine reproductive and respiratory syndrome antigens serving as a detection line, and coated with goat anti-chicken antibodies serving as a quality control line; a sample adding hole is formed in the shell and corresponds to the sample pad; and window holes are formed and correspond to the detection line and the quality control line on the nitrocellulose film. The porcine reproductive and respiratory syndrome antibody detecting card is high in sensitivity, small in detection intra-batch difference and inter-batch difference, good in repeatability and stable in performance, can quickly detect porcine reproductive and respiratory syndrome antibodies in situ, and has high practical value.

Description

technical field [0001] The invention relates to a detection card, in particular to a detection card for rapid and quantitative detection of porcine blue ear virus antibody in pig serum and a preparation method thereof. Background technique [0002] Pig blue-ear disease, also known as porcine reproductive and respiratory syndrome (Porcinereproductiveandrespiratorysyndrome, PRRS), has been popular since the late 1980s. It was officially named by the International Veterinary Bureau in 1992. It mainly affects pigs, especially sows. Reproductive function, the main clinical features are miscarriage, stillbirth, mummified fetus, weak fetus, dyspnea, and transient skin cyanosis on both ears during the course of the disease, so it is also called blue ear disease. Pig blue-ear disease is a serious infectious disease characterized by pig blue-ear virus, breeding of pregnant sows and pigs of various ages, especially piglet respiratory diseases, and has spread all over the world's majo...

AI Technical Summary

Problems solved by technology

[0005] Similarly, enzyme-labeled kits for detecting porcine blue ear antibodies are also widely used in the market, that is, ELISA enzyme-labeled kits. ELISA kits require enzyme-labeled readers, incubation reaction conditions, plate washing conditions, operating environment and professional technicians. Samples also need complex pre-treatment, such as blood collection, serum separation, etc.; while rapid colloidal gold detection is convenient and does not require professional technicians and working environments, but its detection sensitivity is low, and it can only be qualitative, and the detection range is narrow

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