Method for separating phycobiliprotein from laver processing wastewater

A technology for processing wastewater and phycobiliproteins, which is applied in the field of separation of phycobiliproteins, can solve problems such as increasing environmental burdens and wasting resources, and achieve the effects of repeated utilization, improved utilization, and low cost

Active Publication Date: 2016-05-25
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the chromaticity of "red water" exceeds the standard, direct discharge will not only waste resources, but also increase the burden on the environment

Method used

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  • Method for separating phycobiliprotein from laver processing wastewater
  • Method for separating phycobiliprotein from laver processing wastewater
  • Method for separating phycobiliprotein from laver processing wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Measure 200ml of laver processing wastewater, filter out laver residue, sludge and other impurities with a sieve to obtain a crude protein solution, put it in a 250ml blue cap bottle, and put it in a 4°C refrigerator for later use.

[0032] 2. According to the formula protein (Pro) = OD 562 / 0.0157, the calculated protein content in the crude protein solution is 4.39mg / L, and adopts the absorption spectrum of the crude protein solution extracted by ultraviolet spectrophotometer at room temperature, figure 1 It is the absorption spectrum of the crude protein solution at 400-800nm. There are many absorption peaks at 400-450nm in the figure, indicating that there are many impurities.

[0033] 3. Take 2ml of Q-Sepharose (Q-selpharose) stored in alcohol and fill it into a 50ml centrifuge tube, add 10 times the volume of distilled water, and shake to disperse the Q-Sepharose (Q-selpharose) filler. Let stand to make the Q-sepharose filler sink. Aspirate the supernatant. ...

Embodiment 2

[0040] 1. Measure 1L of seaweed processing wastewater, filter out seaweed residue, sludge and other impurities with a filter cloth to obtain a crude protein solution, put it in a 1L round bottle with a vent at the bottom, and store it in a 4°C refrigerator for later use.

[0041] 2. According to the formula protein concentration (mg / L) = OD 562 / 0.0157, the calculated protein content in the crude protein liquid is 4.39mg / L.

[0042]3. Take 20ml of Q-Sepharose (Q-selpharose) stored in alcohol and stuff it into a 500ml sampling bottle, add 10 times the volume of distilled water, shake to make the Q-Sepharose (Q-selpharose) filler disperse open. Let stand to allow the filling to sink. Aspirate the supernatant. Repeat three times to fully remove the alcohol.

[0043] 4. At 25°C, add the Q-selpharose filler that has been washed off the alcohol into the crude protein solution, and blow the Q-selpharose filler with air at the bottom to make the algae The biliprotein is in full c...

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Abstract

The invention discloses a method for separating phycobiliprotein from laver processing wastewater. The method comprises the following steps: (1) filtering laver processing wastewater to remove impurities to obtain laver processing wastewater without impurities; (2) adsorbing phycobiliprotein in the laver processing wastewater without impurities with ion exchange packing so as to obtain phycobiliprotein adsorbed ion exchange packing; and (3) eluting the phycobiliprotein adsorbed ion exchange packing with 0.1-0.5M of sodium chloride-PBS buffer solution to obtain purified phycobiliprotein. According to the method, purified high-active-value phycobiliprotein can be reclaimed from laver processing wastewater, so that the aim of waste utilization can be achieved; meanwhile, environmental pollution caused by direct drainage of laver processing wastewater can be reduced due to extraction of the phycobiliprotein.

Description

technical field [0001] The invention relates to a method for separating phycobiliproteins, in particular to a method for separating phycobiliproteins from laver processing wastewater. Background technique [0002] Laver has important economic value and is one of the main economic seaweeds in my country. The main varieties of laver cultivated in my country are Porphyra altar and Porphyra variegata, among which Porphyra altar is mainly cultivated in the south of the Yangtze River (Fujian Province and Guangdong Province), and Porphyra variegata is mainly cultivated in the north of the Yangtze River (Jiangsu Province and Shandong Province, mainly in the Nantong, Yancheng and Lianyungang sea areas). According to statistics, the annual output of laver in 2006 was about 1.8×10 4 tons of dry product, with an estimated annual output value of about US$1.3 billion (FAO, 2006). [0003] Phycobiliprotein is a water-soluble pigment protein with unique absorption and fluorescence emissio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/18
CPCC07K14/795
Inventor 王广策牛建峰黄爱优
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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