Microbial organic fertilizer for preventing and controlling strawberry red core disease, and production method thereof
A technology of microbial organic fertilizer and production method, which is applied in the treatment of biological organic parts, the preparation of organic fertilizers and organic fertilizers, etc. Significant effect, significant prevention and treatment effect
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Embodiment 1
[0028] Embodiment 1: be used for the preparation of the microbial organic fertilizer of preventing and treating strawberry red column disease:
[0029] 1. Materials:
[0030] 1.1 Bacterial strains: Streptomyces shandongensis is Streptomyces shandongensis recorded in the paper "Preliminary Study on Streptomyces hyperthermia 24#" (Li Deshun, Acta Microbiology 2004, 5th issue) of Shandong University; Strain with catalog number CICIMB1594.
[0031] 1.2 Medium:
[0032] LB medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH7.2, sterilized at 121°C for 20min.
[0033] Gaoshi No. 1 medium: by mass percentage, soluble starch 20‰, KNO 3 1‰, NaCl0.5‰, K 2 HPO 4 0.5‰, MgSO 4 0.5‰, FeSO 4 0.01‰, agar 20g, water 1000ml, pH7.2-7.4.
[0034] Fermentation medium: bran 10g / L, bean cake powder 6g / L, corn flour 10g / L.
[0035] 2. Preparation method:
[0036] (1) Mix chicken manure and mushroom dregs according to the mass ratio of 45:50, and carry out composting and fer...
Embodiment 2
[0043] Embodiment 2: the prevention and treatment effect of microbial organic fertilizer of the present invention to strawberry red column disease
[0044] Select the diseased field of strawberry red column disease, and design three treatments: treatment 1: control (contrast CK is the application of common organic fertilizer); treatment 2: application of compound Trichoderma (Guangzhou Microbiology Technology Co., Ltd.); treatment 3: application of The microbial organic fertilizer prepared in Example 1 of the present invention.
[0045] Each treatment was repeated 3 times, and the application rate was 50Kg / mu, and statistical analysis was carried out after 30 days of application.
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