Traditional Chinese medicine composition for treating liver cancer and preparing method and application thereof
A technology for treating liver cancer and a composition is applied in the field of traditional Chinese medicine composition for treating liver cancer and its preparation, which can solve the problems of great harm to the human body, and achieve the effects of easy storage, small dosage and fast absorption.
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[0053] 2.2.2 Preparation of reference solution
[0054] Accurately weigh an appropriate amount of the reference substance of radix glycoside, add methanol to dissolve it and set the volume in a 10ml volumetric flask to prepare a reference substance solution containing 0.250 mg of radix glycoside per milliliter.
[0055] 2.2.3 Preparation of the test solution
[0056]Precisely draw 10ml of samples under each process, put them in a 25ml volumetric flask, add an appropriate amount of methanol, sonicate for 15 minutes and set the volume to the mark, filter, discard the initial filtrate, and take the subsequent filtrate as the test solution.
[0057] 2.2.4 Measurement results
[0058] Draw 10 μL of the reference substance solution and the solution of the test solution under each process, inject it into the liquid chromatograph, measure it according to the above-mentioned chromatographic conditions, and calculate the content of sheganoside in the sample by the external standard met...
Embodiment 1
[0091] The formula of Chinese medicine composition of the present invention is as follows:
[0092] Shegan 30g, Astragalus 50g, Polygonum cuspidatum 15g, Chenpi 15g, Polyporus 30g, Plantago 15g, Yinchen 10g, Jiaohawthorn 20g, Honeycomb 10g, Qingpi 5g.
[0093] The preparation method of Chinese medicine composition of the present invention is:
[0094] Take 30g Shegan, 50g Astragalus, 15g Polygonum cuspidatum, 15g Chenpi, 30g Polyporus, 15g Plantago, 10g Capillary, 20g Jiaohawthorn, 10g Honeycomb, 5g Qingpi, add water and boil twice, the first time for 2 hours, the second time For 1.5 hours, combine the two filtrates, concentrate to a certain amount, carry out alcohol precipitation with 50% ethanol by volume, let it stand for 48 hours, extract the supernatant, recover the ethanol until it has no alcohol smell, filter, add distilled water to 100ml, Stir evenly, subpackage, and sterilize.
Embodiment 2
[0096] The formula of Chinese medicine composition of the present invention is as follows:
[0097] Shegan 27g, Astragalus 45g, Polygonum cuspidatum 13g, Chenpi 13g, Polyporus 33g, Plantago 17g, Yinchen 11g, Jiaohawthorn 18g, hive 9g, Qingpi 4g.
[0098] The preparation method of Chinese medicine composition of the present invention is:
[0099] Take 27g of Shegan, 45g of Astragalus, 13g of Polygonum cuspidatum, 13g of Chenpi, 33g of Polyporus, 17g of Plantago, 11g of Yinchen, 18g of Jiaohawthorn, 9g of Honeycomb, 4g of Qingpi, add water and boil twice, the first time for 2 hours, the second For 2 hours, combine the two filtrates, concentrate to a certain amount, carry out alcohol precipitation with 55% ethanol by volume, let it stand for 48 hours, extract the supernatant, recover the ethanol until it has no alcohol smell, filter, add distilled water to 100ml, Stir evenly, subpackage, and sterilize.
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