A kind of hardening and transplanting method of soil ginseng aseptic seedling

Abstract

The invention discloses an acclimatization and transplantation method for talinum crassifolium aseptic seedlings. The method specifically includes the following steps that after being subjected to high-pressure sterilization, aseptic soil is cooled to 25 DEG C for use; the sterilized soil is slightly added to a culture bottle of the rooted talinum crassifolium aseptic seedlings through aseptic tweezers, the bottle is slightly covered with a bottle cap, after illumination culture is conducted for 10 days, the bottle cap is opened, after continuous culture is conducted for 7 days at the temperature of 20-25 DEG C, the aseptic seedlings are directly transplanted in a field just like transplantation of seedlings obtained after common seeds germinate along with a culture medium and the added aseptic soil, the aseptic seedlings are covered with a sunshade net with the sunshade degree larger than 70%, the sunshade net can be removed after 15 days, and the seedling emergency rate is 90% or above. The acclimatization and transplantation method without culture medium cleaning is similarly suitable for aseptic seedlings such as crotalaria sessiiflora, kalanchoe blossfeldiana and osmunda japonica, and the purposes that the aseptic seedlings of 5 mm or above can be acclimatized and transplanted, water resources are saved, environmental pollution of culture medium waste is avoided, operating time and labor are reduced, the seedling emergency rate is increased, and production cost is reduced are achieved.

Description

technical field [0001] The invention relates to the fields of tissue culture, breeding and cultivation of plants, etc., and specifically refers to a seedling hardening and transplanting method of aseptic soil ginseng seedlings, which can improve the transplanting seedling rate of the aseptic soil ginseng seedlings. Background technique [0002] Soil ginseng ( Talinum paniculatum ), also known as soil ginseng, false ginseng, ginseng vegetables, ginseng grass, soil Korean ginseng, etc., is a perennial herb of the family Portulaca oleracea. The roots and leaves are edible, can be fried, made soup, rinsed, stewed, and used as medicine and vegetables. In the leaves of ginseng, the oxalic acid content is high, rich in calcium, phosphorus, iron, ascorbic acid, thiamine and other nutrients; its root is similar to ginseng, contains carbohydrates, can be stewed or soaked in wine; young leaves, young shoots Edible, but also cold, stir-fried, fresh and tender taste. Soil ginseng is a...

AI Technical Summary

Problems solved by technology

[0003] In the research process of functional genes and breeding, tissue culture is a very important means. However, when the aseptic seedlings of soil ginseng cultivated by tissue culture are domesticated and hardened, the stems and leaves are tender and brittle, and the stems are easy to break during the process of cleaning the medium. At the same time, domestication and hardening of seedlings with common methods such as perlite can easily lead to water shortage or disease death of tender seedlings, and the seedling rate is low, generally around 50%. height), it is more difficult to harden seedlings successfully

Moreover, the conventional seedling hardening and transplanting operation process is relatively slow and complicated, and consumes more time and manpower, which is not conducive to the breeding and cultivation of native ginseng.

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