Colloidal gold chromatographic test strip for detecting cytokeratin 19 (CK19) and preparation method thereof

Abstract

The invention relates to a colloidal gold chromatographic test strip for cytokeratin 19 (CK19) and a preparation method thereof. The colloidal gold chromatographic test strip comprises a base plate, a sample pad, a combination pad, a nitrocellulose membrane, and a water absorbing pad. The sample pad, combination pad, nitrocellulose membrane, and water absorbing pad are sequentially pasted on one end of the base plate. The test strip is characterized in that the combination pad is made of a glass fiber membrane, a CK19 monoclone antibody-colloidal gold conjugate is painted on the glass fiber membrane; a detection T line and a quality control C line are embedded in the nitrocellulose membrane; the T line is the polyclonal antibody of CK19, and the C line is the corresponding second antibody of the CK19 monoclonal antibody. The prepared colloidal gold chromatographic test strip has the advantages of user-friendliness, fastness, high sensitivity, and good specificity, can be used during the operation, can provide guides for the post operation scheme, and has an important clinical meaning.

Description

technical field [0001] The invention relates to the technical field of immune detection and analysis, in particular to a colloidal gold chromatography test strip for detecting cytokeratin 19 (CK19) in lymphoid tissue, tumor tissue lysate or body fluid, and tumor cells and a preparation method thereof . Background technique [0002] With the rapid changes in lifestyle, the probability of modern people suffering from cancer is increasing, and its high morbidity and mortality have become the main cause of death in urban and rural populations. Surgery is one of the most common methods of cancer treatment. With the continuous deepening of people's understanding of cancer treatment, the mode of surgical treatment has gradually changed from "maximum tolerable treatment" to "minimum effective treatment", paying more attention to the quality of life of patients after surgery. [0003] The sentinel lymph node refers to one or several lymph nodes first reached by cancer cells that me...

AI Technical Summary

Problems solved by technology

Paraffin section is the gold standard for judging whether cancer cells have metastasized. This method usually takes 3 to 5 days to get the results, and it cannot quickly provide information on whether the sentinel lymph has metastasized during the operation.

If this method is adopted, the patient will have to undergo two operations, which will cause great pain to the patient and delay follow-up treatment at the same time.

At the same time, the slicing method also has common shortcomings: 1. The number of sentinel lymph nodes is generally as many as 10 or more. In order to save manpower, generally only a few are randomly selected for detection, which is likely to cause missed detection

2. The degree of tissue utilization is low. Usually, only a few sections are cut for staining analysis, and the rest are discarded. If the size of the metastases is small, it is easy to cause missed detection

3. The result is not an objective standard

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