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Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta

The invention relates to a technology for in vitro culture of kiwifruit in jujube, which is applied to the field of storage of kiwifruit in jujube pollen and detection of viability of in vitro culture. Long, easy to handle, low storage temperature effect

Active Publication Date: 2016-05-11
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a method for detecting actinidia jujube pollen storage and in vitro culture activity, the storage temperature of the method is lower than the traditional method, and the pollen activity is kept for a long time, which can solve the problem that the pollen activity declines significantly after one year of storage Problems, adapting to the detection method of pollen in vitro culture activity of Actinidia jujube can solve the problems of time-consuming, labor-intensive, large error, complicated operation and not accurate enough in the existing method

Method used

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  • Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta
  • Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta
  • Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A method for detecting the pollen storage and in vitro culture activity of Actinidia jujube:

[0047] S11, collecting the flower buds of Actinidia jujube at the big bud stage and taking out the anthers;

[0048] S12, drying and sieving the anthers to collect pollen;

[0049] S13, sealing the collected pollen and storing it at a low temperature of -85~-75°C;

[0050] S14. Take out the pollen stored at low temperature, place it at room temperature for rewarming, spread the pollen evenly on the solid medium for cultivation, and then examine it under a microscope and count the pollen germination rate.

Embodiment 2

[0052] A method for detecting the pollen storage and in vitro culture of Actinidia jujube:

[0053] S21. At 9:00 in the morning on a sunny day, collect the flowers of Actinidia jujuba that are about to open in the big bud stage, and pick out the anthers with tweezers;

[0054] S22. Put the anther in a sulfuric acid paper bag, and then place the sulfuric acid paper bag in a desiccator filled with discolored silica gel. The drying temperature in the desiccator is 20-25° C., and the drying time is 20 hours, so that the anther Moisture content reaches 6%;

[0055] S23, through a 60-mesh sieve to collect pollen;

[0056] S24. Put the collected pollen into a sealed glass bottle or centrifuge tube and store it at -85°C;

[0057] S25, taking out the pollen stored at low temperature, and rewarming at room temperature for 5 minutes;

[0058] S26. Evenly sow rewarmed pollen on a solid medium for cultivation, then examine under a microscope and count the pollen germination rate;

[0059...

Embodiment 3

[0062] A method for detecting the pollen storage and in vitro culture activity of Actinidia jujube:

[0063] S31. At 10:30 in the morning on a sunny day, collect the flowers of Actinidia jujuba that are about to open in the big bud stage, and pick out the anthers with tweezers;

[0064] S32. Put the anther in a sulfuric acid paper bag, and then place the sulfuric acid paper bag in a desiccator filled with discolored silica gel. The drying temperature in the desiccator is 20-25° C., and the drying time is 28 hours, so that the anther Moisture content reaches 4.5%;

[0065] S33, cross 80 mesh sieves to collect pollen;

[0066] S34. Put the collected pollen into a sealed glass bottle or centrifuge tube and store it at -75°C;

[0067] S35, taking out the pollen stored at low temperature, and rewarming at room temperature for 10 minutes;

[0068] S36. Evenly sow rewarmed pollen on a solid medium for cultivation, then examine under a microscope and count the pollen germination ra...

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Abstract

The invention relates to the field of plant pollen preservation and in-vitro culture vitality detection and particularly relates to a preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta. The method comprises the steps: picking flower buds of the Actinidia arguta at a big bud stage and taking out anthers; drying the anthers, and carrying out screening, so as to collect pollen; sealing the collected pollen, and carrying out cryopreservation at the temperature of -85 DEG C to -75 DEG C; taking out the cryo-preserved pollen, and rewarming the cryo-preserved pollen at room temperature; uniformly spreading the rewarmed pollen onto a solid culture medium, carrying out culturing, and then, carrying out microscopy under a microscope, and counting the germinating rate of the pollen. According to the preservation method, the vitality of the pollen is still preserved good after the pollen is preserved for a long time, so that the pollen in-vitro culture detection method can be applied to the vitality detection on the pollen of the Actinidia arguta and is simple, rapid and accurate.

Description

technical field [0001] The invention relates to the field of plant pollen storage and in vitro culture activity detection, in particular to a method for the storage of actinidia jujube pollen and in vitro culture activity detection. Background technique [0002] Actinidia arguta (Sieb.&Zucc) Planch.exMiq.), also known as Actinidia arguta (Sieb. & Zucc) Planch.exMiq., also known as Actinidia arguta, kiwi pear, rattan melon, and vine pear, belongs to the family Actinidia, and Actinidia is a large deciduous vine. Its fruit is small, smooth, and edible as a whole. It is one of the most cold-resistant species in the genus Actinidia. Jujube kiwifruit enjoys the reputation of "the king of fruits". It is favored by consumers for its excellent quality, flavor, rich nutritional content, high medical and health care effects, and good processing suitability. Actinidia jujube is a promising small berry tree species in many regions of the world, especially in cold regions. [0003] Conv...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5097
Inventor 秦红艳艾军许培磊刘迎雪王振兴杨义明范书田赵滢王春伟李晓艳张宝香王广富
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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