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New Purpureocillium sp strain F1 and application thereof

A technology of Porphyrosporium and strains, applied in the field of new strains of Porphyrospora and its application in the adsorption of heavy metals, to achieve the effect of enriching germplasm resources

Inactive Publication Date: 2016-05-11
TAIYUAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on Porphyrospora has only seen reports on the control of nematodes and pests by Porphyrospora in my country, but there have been no reports on the use of Porphyrospora as a heavy metal adsorbent.

Method used

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  • New Purpureocillium sp strain F1 and application thereof
  • New Purpureocillium sp strain F1 and application thereof
  • New Purpureocillium sp strain F1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] Trapping isolation of new strains of Porphyrospora:

[0046] (1) The sludge filtered from the sewage of Yangjiabao Sewage Treatment Plant in Taiyuan City was put into a ziplock bag and dried naturally in the laboratory.

[0047] (2) Resistance to heavy metal Cd 2+ Trapping separation: at a concentration of 2mmol / LCd 2+ PB liquid medium (cut potatoes 200g into pieces, add water and boil for 20min, filter the filtrate and add 20g of glucose, set the volume to 1000mL, autoclave at 121°C for 20min), add 1g of dried sludge and place in a shaker at 30°C, 180r / min shaking culture for 3 days.

[0048] (3) Take the suspension in (2) and do concentration gradient dilution to 10 -7 , draw 10 -5 、10 -6 、10 -7 Each 150μl of the bacterial suspension was coated on the Cd-containing 2+ PDA (cut potatoes 200g into pieces, add water and boil for 20min, filter the filtrate, add glucose 20g, agar powder 15g, dilute to 1000mL, autoclave at 121°C for 20min) on a plate, place it upside...

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Abstract

The invention relates to a new Purpureocillium sp strain F1 and application thereof, in particular to the new Purpureocillium sp strain F1 separated from sludge subjected to sewage filtration in the sewage treatment plant of Yang Jiabao, Taiyuan, Shanxi Province, is preserved in China General Microbiological Culture Collection Center in December 17th, 2015, and has the classification name of Purpureocillium sp and the preservation number of CGMCC No.11813. The new Purpureocillium sp strain has wide application prospects on the aspect of heavy metal adsorption.

Description

technical field [0001] The invention relates to a new microorganism strain and its application, in particular to a new porphyrosporium strain and its application in absorbing heavy metals. Background technique [0002] Water is the source of life, March 22 is the "World Water Day" established by the United Nations. In 2015, the "World Water Resources Development Report" issued by the United Nations pointed out that the world today is facing the common challenge of water resource crisis. Once water is polluted, it cannot be used for drinking, bathing, industrial or agricultural purposes. Poor water quality can damage human health and lead to degradation of ecosystem services. my country is a country with serious water pollution in the world, especially the accumulation of heavy metals, which has become one of the important factors of water pollution. [0003] At present, there have been many methods of removing heavy metals in water at home and abroad, such as chemical repa...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/20
CPCC02F3/34C02F2101/20C12N1/145C12R2001/645
Inventor 乔宏萍王青燕平梅武晓英吴丽华郝雪峰
Owner TAIYUAN NORMAL UNIV
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