A kind of vaccine composition against foot and mouth disease and its preparation method and application

A vaccine composition and composition technology, applied in the direction of biochemical equipment and methods, methods based on microorganisms, medical preparations containing active ingredients, etc., can solve the problems of short validity period, incompletely killed or fully attenuated viruses, and pollution And other issues

Active Publication Date: 2019-03-05
PU LIKE BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also the following disadvantages: 1. The virus must be produced in highly sealed equipment to prevent contamination of the surrounding environment; during the production process, the virus may not be completely killed or fully attenuated, which will cause the vaccine to contain highly toxic pathogens. The disease substance, which in turn causes the disease of the immunized animals and causes the disease to spread on a large scale
2. The validity period is very short, and often need to be frozen, which increases the difficulty of promoting the use of vaccines in rural areas
3. Inactivated vaccine immunity is indistinguishable from FMD-infected animals, which limits the export of animals
4. Production depends on FMDV, so FMDV cannot be completely eliminated
However, the low replication ability and virus stability of type A foot-and-mouth disease epidemic virus make it difficult to form virus-like particles, and the stability is poor, which cannot induce sufficient immunity in animals

Method used

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  • A kind of vaccine composition against foot and mouth disease and its preparation method and application
  • A kind of vaccine composition against foot and mouth disease and its preparation method and application
  • A kind of vaccine composition against foot and mouth disease and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Protein expression of foot and mouth disease with sequences SEQ ID NO.1, 3, 5, 7, 9, 11, 13, 15, 17

[0051] 1. Preparation of foot-and-mouth disease gene fragments used as templates

[0052] Respectively the O-type foot-and-mouth disease VP0, VP3, VP1 gene shown in the sequence SEQ ID NO.1, 3, 5, the Asian type 1 foot-and-mouth disease VP0, VP3, VP1 gene shown in the sequence SEQ ID NO.7, 9, 11, the sequence SEQ ID NO. The full lengths of A-type FMD VP0, VP3, and VP1 genes represented by ID NO.13, 15, and 17 were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. The full lengths of the synthesized gene fragments are 909bp, 660bp, 639bp, 909bp, 657bp, 627bp, 909bp, 663bp, 636bp, respectively. The foot-and-mouth disease gene template of the present invention is prepared on the basis of the artificially synthesized foot-and-mouth disease gene fragment.

[0053] 2. Construction of foot-and-mouth disease gene expression vector

[0054] FMD gene template synthesiz...

Embodiment 2

[0068] Preparation of vaccine composition against foot-and-mouth disease

[0069] Get the O-type foot-and-mouth disease virus-like particles prepared in Example 1, the Asian type I foot-and-mouth disease virus-like particles and the A-type foot-and-mouth disease virus-like particles, and slowly add them to the adjuvant, and the process of adding is constantly stirring with a rotating speed of 800rpm emulsifier for 12min, mixing Evenly and stored at 4°C, it becomes the vaccine composition against foot-and-mouth disease. See Table 3 for specific ratios. The adjuvants suitable for the present invention may be adjuvants known to those skilled in the art. In this example, the adjuvant ISA 206 (Seppic, France) was selected.

[0070] The composition ratio of the vaccine composition against foot-and-mouth disease of table 3

[0071]

Embodiment 3

[0073] Immunogenicity test of vaccine composition against foot-and-mouth disease

[0074] 1. Immunization procedure

[0075] 30 6-month-old healthy cattle that were negative for the above three antibodies were screened using the FMD O-type antibody ELISA detection kit, the Asian I-type ELISA antibody detection kit and the A-type antibody ELISA detection kit, and were randomly divided into 6 groups. 5 heads per group. Groups 1-5 are vaccine 1, vaccine 2, vaccine 3, vaccine 4, and vaccine 5 immunization groups prepared in Example 2 of the present invention, respectively, and group 6 is a PBS control group. The way of immunization in the immunization group was intramuscular injection of 1 ml in the neck, and the control group was immunized with the same amount of PBS. Blood was collected from each cow before vaccine immunization and every week after immunization until 21 days after immunization.

[0076] 2. Detection of antibody level

[0077] The collected sera were tested f...

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Abstract

The invention relates to the field of veterinary biological products. More specifically, a type A foot-and-mouth disease virus-like particle, an anti-foot-and-mouth disease vaccine composition, a preparation method thereof, and an application for preventing and / or treating infection-related diseases caused by foot-and-mouth disease virus are disclosed. The vaccine composition of the present invention comprises type O foot-and-mouth disease virus-like particles and / or Asian type I foot-and-mouth disease virus-like particles and / or type A foot-and-mouth disease virus-like particles and adjuvants.

Description

technical field [0001] The invention relates to the field of veterinary biological products. More specifically, the present invention relates to a vaccine composition against foot-and-mouth disease. The present invention also relates to the application of the vaccine composition for preventing and / or treating diseases related to infection caused by foot-and-mouth disease virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease that can spread quickly and over long distances. It is the most contagious disease among mammals, and the infection of artiodactyls will cause significant economic losses worldwide. Animals affected by FMD include cattle, sheep, goats and pigs. The causative agent, foot-and-mouth disease virus (FMDV), is an aphthous sore virus of the picornavirus family. The virus is divided into 7 serotypes (A, O, C, Asia 1, SAT1, SAT2, and SAT3), of which the A-type foot-and-mouth disease virus is more widespr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/04A61K39/135A61P31/14C12R1/93
Inventor 张许科袁于人孙进忠陈红英肖燕田克恭
Owner PU LIKE BIO ENG
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