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Technique for separating and purifying succinic acid from microbial fermentation liquid

A microbial fermentation liquid, separation and purification technology, applied in the field of separation and purification of organic acids, can solve the problems of low purity, high energy consumption, low yield, etc., and achieve the effect of high purity, simple process steps, and high yield

Active Publication Date: 2016-05-11
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention overcomes the disadvantages of low yield, low purity, high energy consumption and high cost in the existing process

Method used

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  • Technique for separating and purifying succinic acid from microbial fermentation liquid
  • Technique for separating and purifying succinic acid from microbial fermentation liquid
  • Technique for separating and purifying succinic acid from microbial fermentation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: The succinic acid-producing microorganism EscherichiacoliNZN111 fermented liquid was filtered and centrifuged to remove bacterial cells and insoluble solid particle impurities, and the supernatant fermented liquid was taken for later use. Take 10mL supernatant fermentation broth (initial succinic acid concentration is 101.77g / L) into a clean and dry test tube, add 4.0g urea to the test tube, heat in a water bath until the solid in the test tube is completely dissolved, and then place it at 4°C for 8h , filtered and dried to obtain 2.08 g of needle-like solid. Dissolve 2.08g of needle-like solid in a beaker containing 100mL of anhydrous methanol, weigh 2.1g of NaOH and dissolve it in another beaker of 100mL of anhydrous methanol, then pour the NaOH alcohol solution into the beaker containing the needle-like solid alcohol The solution was mixed in a beaker, a large amount of white solid precipitated out, filtered and dried to obtain a white solid. Dissolve the...

Embodiment 2

[0033]Example 2: The succinic acid-producing microorganism EscherichiacoliNZN111 fermented liquid was filtered and centrifuged to remove bacterial cells and insoluble solid particle impurities, and the supernatant fermented liquid was taken for later use. Take 50mL of supernatant EscherichiacoliNZN111 fermentation liquid (initial concentration of succinic acid 101.77g / L) in a clean and dry test tube, add 20.0g of urea to the test tube, sonicate until the solid in the test tube is completely dissolved, then place it at room temperature for 8h, filter Drying yielded 10.50 g of needle-like solids. Dissolve the needle-like solid in a beaker containing 400mL of absolute ethanol, weigh 10.50g NaOH and dissolve it in another beaker containing 750mL of absolute ethanol, then pour the NaOH alcohol solution into the beaker containing the needle-like solid alcohol solution Mixing in the medium, a large amount of white solid precipitated out, filtered and dried to obtain a white solid. D...

Embodiment 3

[0034] Example 3: The succinic acid-producing microorganism EscherichiacoliNZN111 fermented liquid was filtered and centrifuged to remove bacterial cells and insoluble solid particle impurities, and the supernatant fermented liquid was taken for later use. Take 100mL of the supernatant EscherichiacoliNZN111 fermentation liquid (the initial concentration of succinic acid is 101.77g / L) in a clean and dry beaker, add 40.0g of urea to the beaker, heat in a water bath until the solid in the beaker is completely dissolved, and then place it at room temperature for 12h , filtered and dried to obtain 20.29 g of needle-shaped solid. Dissolve the needle-like solid in a beaker containing 1000mL of absolute ethanol, weigh 20.30g NaOH and dissolve it in another beaker containing 1500mL of absolute ethanol, then pour the NaOH alcohol solution into the beaker containing the needle-like solid alcohol solution After mixing in a beaker, a large amount of white solid precipitated out, which was ...

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Abstract

The invention discloses a technique for separating and purifying succinic acid from a microbial fermentation liquid by cocrystallization. The method comprises the following steps: adding a proper amount of urea into a pretreated succinic acid fermentation liquid, and carrying out cocrystallization to obtain a succinic acid-urea adduct; and precipitating a sodium succinate solid by utilizing the acid-base neutralization reaction and the property that the sodium succinate is insoluble in alcohols, converting the sodium succinate into succinic acid by using a strongly-acidic cation exchange resin, carrying out reduced pressure evaporation, and drying to obtain the succinic acid of which the purity is greater than 99%. The technique for separating and purifying succinic acid from a microbial fermentation liquid is simple and green, and has the advantages of low production cost, high quality and environment friendliness.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to the separation and purification of organic acids in microbial fermentation liquid. technical background [0002] Succinic acid, also known as succinic acid, molecular formula: C 4 h 6 o 4 , Molecular weight: 118.09, is a natural dibasic organic carboxylic acid that widely exists in the human body, animals, plants and microorganisms. As one of the C4 platform compounds, it has a wide range of applications in pharmaceutical, food, chemical and agricultural industries. At the same time, as a bulk raw material chemical, the market demand is quite large. [0003] At present, the source of succinic acid is mainly synthesized by catalytic hydrogenation of maleic anhydride in petroleum raw materials. With the increasingly severe environmental and energy problems, this production method does not meet the requirements of sustainable development. The production of succinic acid by biot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/487C07C51/42C07C55/10
CPCC07C51/42C07C51/487C07C55/10
Inventor 朱笃肖依文张志斌汪涯常军吴文婷
Owner JIANGXI SCI & TECH NORMAL UNIV
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