Application of bupleurum polysaccharide in preparation of drugs used for preventing and treating diabetic nephropathy
A technology for diabetes medicine and diabetic nephropathy, which is applied in the field of traditional Chinese medicine and can solve problems such as the efficacy of Bupleurum polysaccharides that have not been reported
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Embodiment 1
[0051] Take 100g of Bupleurum herb, extract by cold soaking with 95% ethanol, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and the supernatant is washed with trichloro Remove free protein with acetic acid, centrifuge, dialyze the supernatant with water for 3 days, concentrate the dialysate to a small volume, add ethanol to 80% alcohol content, centrifuge, precipitate and freeze-dry to obtain the total polysaccharide, the product yield is more than 3%, the polysaccharide content More than 65%.
[0052]The Bupleurum medicinal material can be selected from Bupleurum chinense DC or Bupleurumsmithiivar.parvifolium.
Embodiment 2
[0054] Fifty-six C57BL / 6 mice (8 weeks old) were randomly divided into 6 groups (groups A, B, C, D, E, and F) according to body weight: group A was the normal control group, and group B was given Bupleurum polysaccharide alone. Medicine group (normal mice were given Bupleurum polysaccharide, and the dosage was 60mg·kg -1 ), group C is the model group, group D is the model group + Bupleurum polysaccharide 30mg·kg -1 Dosage group, group E is the model group + Bupleurum polysaccharide 60mg·kg -1 Dosage group, F group is the model group + glibenclamide positive group (7.2mg·kg -1 ), each group ranging from 8-12. Taking the day before modeling as Day-1, after the mice were fasted overnight for 15 hours, the models were established in groups C, D, E, and F, and the dose of STZ (Streptozotocin, streptozotocin) 100mg / kg was used for two consecutive days Intraperitoneal injection (0.1mL·10g -1 ). The mice in groups A and B were injected with the same amount of normal saline to sim...
Embodiment 3
[0056] The renal tubular cells were seeded, adhered to the wall overnight, and starved for 24 hours. The experiment was divided into 10 groups. They were: Normal group (normal control group); TGF-β group (model group); SB group (single plus TGF-β inhibitor); BPs20 group (single plus Bupleurum 20 μg / mL group); BPs40 group (single plus Bupleurum 40μg / mL group); BPs80 group (bupleurum 80μg / mL alone group); SB-TGF group; BPs20-TGF group; BPs40-TGF group; BPs80-TGF group. After 48 hours of intervention in each group, the cells were fixed with 4% paraformaldehyde, and the cell morphology was observed and photographed under an inverted microscope.
[0057] The results of animal experiments show that Bupleurum polysaccharides can improve the body weight of diabetic model animals, significantly reduce blood sugar and urine sugar levels, significantly improve insulin secretion, lower serum creatinine levels, reduce urine microalbumin secretion, and lower kidney index. The results of pa...
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