Novel human-like collagen haemostatic dressing

A technology of human-like collagen and hemostatic dressings, which is applied in medical science, drug delivery, bandages, etc., can solve the problems of platelets and other hemostatic components, poor pulling ability and wound repair ability, eosinophilia, and pathogens carried by animals. , to achieve the effects of increasing the ion assisting effect, easy process amplification, and stable product quality

Active Publication Date: 2016-05-04
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, for absorbable collagen-based wound hemostatic materials, because collagen is derived from animal skin, tendons, etc., it is a kind of heterologous substance to the human body, which can cause allergic and other adverse reactions, manifested as fever, eosinophilia, etc. Cells increase, causing wound infection; hemostatic speed is slow, and the pulling ability of hemostatic components such as platelets and wound repair ability are not good
In addition, heterologous animal collagen also has some potential risks of carrying pathogens in animals

Method used

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  • Novel human-like collagen haemostatic dressing
  • Novel human-like collagen haemostatic dressing
  • Novel human-like collagen haemostatic dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of Human Collagen Hemostatic Dressing Rich in Calcium, Zinc, Iron and Copper

[0024] According to the mass ratio of 100:2:2:1, take an appropriate amount of chelated calcium, zinc, iron, and copper-type human-like collagen respectively, dissolve them in an appropriate amount of deionized water, and prepare a concentration of 10% (w / v) collagen solution 100ml; add 0.05% (w / v) cross-linking agent lysine oxidase 2mL to the above protein solution under stirring, pour into the mold after mixing, and incubate at 25°C for 24h, Then transfer to an ultra-low temperature refrigerator to freeze for 2~4 hours; quickly move the ultra-low temperature frozen sample into a pre-cooled vacuum freeze dryer, vacuum freeze dry for 24~48 hours, take out the package, and sterilize with Co60 irradiation to obtain degradable human collagen Hemostatic dressing. The appearance photos of the prepared novel hemostatic dressing are as follows: figure 1 As shown, the SEM i...

Embodiment 2

[0025] Example 2 Preparation of Human Collagen Hemostatic Dressing Rich in Calcium, Zinc, Iron and Copper

[0026] According to the mass ratio of 100:3:4:1, take an appropriate amount of chelated calcium, zinc, iron, and copper-type human-like collagen respectively, dissolve them in an appropriate amount of deionized water, and prepare a concentration of 5% (w / v) 200ml of collagen solution; under stirring, add 0.4ml of cross-linking agent lysine oxidase with a mass fraction of 0.5% to the above protein solution, mix well and pour into the mold, keep it warm at 20°C for 48h, and then Transfer to an ultra-low temperature refrigerator and freeze for 2-4 hours; quickly move the ultra-low temperature frozen sample into a pre-cooled vacuum freeze dryer, vacuum freeze-dry for 24-48 hours, take out the package, and sterilize it with Co60 irradiation to obtain degradable human collagen for hemostasis dressing.

Embodiment 3

[0027] Example 3 Preparation of Human Collagen Hemostatic Dressing Rich in Calcium, Zinc, Iron and Copper

[0028] According to the mass ratio of 400:10:10:1, take an appropriate amount of chelated calcium, zinc, iron, and copper-type human-like collagen respectively, dissolve them in an appropriate amount of deionized water, and prepare a concentration of 15% (w / v) 200ml of collagen solution; under stirring, add 6mL of cross-linking agent lysine oxidase with a mass fraction of 0.05% (w / v) to the above protein solution, mix well and pour into the mold, at 45°C Insulate for 5 hours, then transfer to an ultra-low temperature refrigerator to freeze for 2-4 hours; quickly move the ultra-low temperature frozen sample into a pre-cooled vacuum freeze dryer, vacuum freeze-dry for 24-48 hours, take out the package, and sterilize it with Co60 irradiation to obtain degradable human resources Type collagen hemostatic dressing.

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Abstract

The invention discloses a novel human-like collagen haemostatic dressing which has good biocompatibility, can be absorbed and is rich in calcium, zinc, copper and iron, and discloses a preparation method of the novel human-like collagen haemostatic dressing. The preparation method comprises the following steps: by taking modified protein obtained by chelating humanized human-like collagen prepared by high-density fermentation with calcium, zinc, copper and iron ions respectively as a raw material as well as taking lysyloxidase existing in mammals as a cross-linking agent, carrying out low-temperature cross-linking reaction and freeze-drying processes to obtain the novel collagen haemostatic dressing which can be used for hemostasis of wounds and postoperative hemostasis. The preparation method disclosed by the invention has the advantages that the raw materials are easy to obtain, the cost is low, the synthetic process is simple and easy to realize, process amplification is easy, and the reproducibility is good; and the human-like collagen haemostatic dressing prepared by the preparation method does not have animal-derived virus hidden danger, has an obvious haemostatic effect, can be used for hemostasis of various wounds and postoperative hemostasis, and can be used for basic research and application of related fields, such as biomedical tissue engineering.

Description

technical field [0001] The invention relates to a novel human-like collagen hemostatic dressing rich in calcium, zinc, copper and iron elements and a preparation method thereof. Background technique [0002] Collagen is a kind of fibrous protein in animals. It is rich in skin, tendons and bones. It has good biocompatibility and is often used as an important raw material for biomedical materials. It can be used as an absorbable wound hemostatic material. The cross-linked porous structure of collagen absorbs blood swelling, forms a coagulation network, seals blood vessel cracks or wounds, and activates platelets to achieve the purpose of hemostasis. At present, for absorbable collagen-based wound hemostatic materials, because collagen is derived from animal skin, tendons, etc., it is a kind of heterologous substance to the human body, which can cause allergic and other adverse reactions, manifested as fever, eosinophilia, etc. Cells increase, causing wound infection; the hemo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0004A61L26/0033A61L26/0061A61L26/009A61L2400/04C08L89/00
Inventor 范代娣米钰郑晓燕马沛惠俊峰
Owner NORTHWEST UNIV(CN)
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