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Method for improving sensitivity and linearity of latex reagent

A sensitivity and latex technology, applied in the field of medical testing, can solve the problems that the accuracy and precision cannot meet the requirements

Inactive Publication Date: 2016-04-20
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The principle of the indirect method is similar to the direct method used by other instruments currently on the market, but due to the fragility of ACA, in order to prevent the inside of the instrument from being blocked, the requirements for the sample are extremely strict, and it needs to be routinely separated and then diluted. Measurement, and the dilution factor of the general biochemical ISE module is about 30 times. Under such a large dilution factor, it is beneficial to the pipeline, but from the perspective of data statistical processing, such measurement will be Enlarge the error in the same proportion, then the accuracy and precision of the measured results cannot meet the requirements

Method used

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  • Method for improving sensitivity and linearity of latex reagent
  • Method for improving sensitivity and linearity of latex reagent

Examples

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Embodiment 1

[0080] Embodiment 1 The preparation of the C-reactive protein detection kit of mixed size latex microspheres

[0081] The main raw materials involved in the kit prepared by adopting the latex microspheres of mixed sizes and sizes of the present invention are as follows:

[0082] 1. Anti-C reactive protein antibody: mouse monoclonal antibody, the titer determined by ELISA method is 1:100000; rabbit polyclonal antibody, the titer determined by ELISA method is 1:50000.

[0083] 2. Latex microspheres: the present invention only adopts polystyrene latex microspheres with carboxyl groups to test, wherein the average particle diameter of the large-diameter latex microspheres is 300-350nm, and the small-particle-diameter latex microspheres The average particle size is 50-100nm.

[0084] The preparation of the main reagent of this embodiment is as follows:

[0085] Reagent R1: 0.2M ammonium chloride buffer solution containing 0.3% PEG8000, 3% sodium chloride, 0.5% sucrose, 0.5% Tween...

Embodiment 2

[0100] The preparation of the main reagents of this embodiment is as follows:

[0101] Reagent R1: 0.2M ammonium chloride buffer containing 0.3% PEG8000, 3% sodium chloride, 0.5% sucrose, 0.5% Tween 20, 0.2% sodium azide, this reagent is a colorless and transparent solution.

[0102] Reagent R2: 0.2M ammonium chloride buffer containing 0.25% anti-C-reactive protein antibody sensitized polystyrene latex microspheres of mixed sizes and sizes, 1.5% sucrose, 0.2% sodium azide. The reagent is a milky white solution.

[0103] The specific preparation steps are as follows:

[0104] 1) Take 1ml (100mg / ml) of latex microspheres with large particle size or small particle size, wash three times with 0.2M, pH5.0 MES solution (2-morpholine ethanesulfonic acid buffer), and disperse;

[0105] 2) Add 0.1ml of 10mg / ml ethyldimethylaminopropylcarbodiimide EDAC solution freshly prepared with 0.2M, pH5.0 MES solution, and react at room temperature for 1 hour;

[0106] 3) Add 0.1ml of 100mg / ml ...

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Abstract

The invention provides a method for improving the sensitivity and linearity of a latex reagent. An adopted detection kit comprises a reagent R1 and a reagent R2. The method is characterized in that the latex reagent is a latex reagent mixed with latexes with large and small particle sizes, and is processed to prepare a C-creative protein latex turbidimetric detection kit, and the kit comprises the reagent R1 and the reagent R2. The method comprises the following steps: (1) directionally coating latex microspheres with large particle sizes with mouse to-be-tested antigen monoclonal antibodies; (2) directionally coating latex microspheres with small particle sizes with rabbit or sheep to-be-tested antigen monoclonal antibodies; (3) mixing the coated latex microspheres with the large particle sizes and the coated latex microspheres with the small particle sizes.

Description

technical field [0001] The invention belongs to the field of medical testing, and in particular relates to a method for simultaneously improving the sensitivity and linearity of latex reagents by mixing large and small particle size latexes. Background technique [0002] C-reactive protein (CRP) is an acute phase-reactive protein that is synthesized by hepatocytes within a few hours after the body is subjected to inflammatory stimuli such as microbial invasion or tissue damage, with a molecular weight of 115-140KD and a half-life of 19 days. β globulin; can be detected in human serum, cerebrospinal fluid, pleural and ascites and other body fluids. The detection of CRP has a wide range of clinical applications, including the diagnosis and differential diagnosis of acute infectious diseases, the monitoring of infection after surgery, the observation of antibiotic efficacy, the detection of the course of disease and the judgment of prognosis. The clinical routine method for me...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 陈青松韩钟林耀文
Owner 浙江夸克生物科技有限公司
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