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Method for detecting antibiotic residues in milk

A technology of antibiotics and milk, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of extracting antibiotic components and low accuracy of test results, and achieve the effect of improving dissolution rate, ensuring accuracy, and improving accuracy

Active Publication Date: 2016-04-20
ZHONGBIAO TESTING HENAN SERVICE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of conventional chemical reagents cannot completely extract the antibiotic components, resulting in low accuracy of the test results

Method used

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  • Method for detecting antibiotic residues in milk
  • Method for detecting antibiotic residues in milk
  • Method for detecting antibiotic residues in milk

Examples

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Embodiment Construction

[0012] The method for detecting antibiotic residues in milk of the present invention will be described in detail below through specific examples. Those skilled in the art should understand that the embodiments described below are only illustrative illustrations of the present invention, and are not intended to limit it in any way.

[0013] Taking milk containing antibiotics after preliminary screening (such as microbial inhibition test paper method) as a sample, detect five kinds of nitroimidazole antibiotics (hydroxymetronidazole, 2-metronidazole, hydroxydimetronidazole, metronidazole , clometrazol) residual specific content, to introduce the implementation process and effect of the present invention.

[0014] 1. Preparation of sample solution A

[0015] 1.1 Extraction steps

[0016] Take 5mL of milk in a centrifuge tube, add 0.3g of polyoxyethylene polyoxypropylene polyether, ultrasonically oscillate for 30 minutes, add 20mL of ethyl acetate, after the dispersion is unifor...

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Abstract

A method for detecting antibiotic residues in milk comprises the following steps: (1) the milk is placed in a centrifuge tube, a demulsifier, ethyl acetate and a phosphate buffered solution are added sequentially, a mixture is subjected to ultrasonic extraction, an extract is centrifuged, and a liquid to be purified is obtained; (2) the liquid to be purified is transferred to a solid-phase extraction column for purification, and an eluent is collected; the eluent is blown with nitrogen in a water bath until the eluent is almost dry, an aqueous acetonitrile solution is added, the materials are mixed on a turbine mixer, a mixture passes through a millipore filter, and a filtrate is to be detected; (3) an LC-MS (liquid chromatography-mass spectrometry) method is used for determination and confirmation, and an external standard method is used for quantification. According to the method for detecting the antibiotic residues in the milk, the accuracy of a detection result can be improved effectively, and beneficial guarantee is provided for food safety.

Description

technical field [0001] The invention relates to a method for detecting antibiotic residues in milk. Background technique [0002] Antibiotic residues in dairy products are a growing concern. In addition to some common inflammatory diseases, dairy cows are particularly susceptible to mastitis, which is often treated with antibiotics by veterinarians. Since general antibiotics can withstand high temperatures, the existing heat sterilization process cannot completely eliminate antibiotic residues in milk. Drinking milk containing trace amounts of antibiotics for a long time will increase the resistance of bacteria in the human body and reduce or completely offset the efficacy of antibiotics. In addition, for certain groups of people, milk containing excessive amounts of antibiotics may also cause allergic reactions to antibiotics. Therefore, the detection of antibiotic residues in milk is of great significance to ensure food safety. [0003] At present, the detection of anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 赵林萍贾松涛李梦雨董瑞静王继美赵市勇黄丽萍魏薇
Owner ZHONGBIAO TESTING HENAN SERVICE
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