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Cotton woven fabric scouring method based on trametes and white-rot fungus combined fermentation crude enzyme liquid

A crude enzyme solution and white rot fungus technology, which is applied in the biochemical treatment of enzymes/microorganisms, plant fibers, textiles and paper making, etc., can solve the problems of not meeting the requirements of wettability, different catalytic conditions, and limited types. , to achieve the effect of improving dyeing performance, reducing the number of washings, and saving the amount of dye

Active Publication Date: 2016-04-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, even if the cotton fabric is treated with a variety of commercial enzymes, its wettability (generally expressed by capillary effect) is very unsatisfactory, and cannot meet the wettability requirements of subsequent dyeing, printing and finishing.
At present, under the premise of my country's traditional industrial transformation and green production, the new pretreatment process of biological enzyme scouring of cotton fabrics in the textile industry has been paid attention to, but the removal of cotton seed hulls on low-quality cotton fabrics is the main technical difficulty in its enzymatic scouring process , it is difficult for a single enzyme preparation to complete the degradation of low-quality cotton fiber companions and cotton seed hulls, and it is difficult to achieve the ideal removal rate and scouring effect. degradation and removal of
[0005] However, due to the high substrate catalytic specificity of enzymes, different types of enzymes can only degrade their different impurity components. Even if the enzyme compound technology is used, due to the different characteristics of enzymes from different biological sources, the catalytic conditions vary greatly. Different enzymes have different optimum reaction pH values ​​and temperatures, as well as pH and temperature stability. The activity and use effects of different enzymes have a considerable relationship with the conditions of use, which increases the difficulty of compounding. Gluease, hemicellulase and ligninase are a combination of a series of enzymes. At present, the types of commercial enzymes are limited and incomplete, so the best synergistic treatment effect cannot be achieved, which is a technical difficulty in the scouring process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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  • Cotton woven fabric scouring method based on trametes and white-rot fungus combined fermentation crude enzyme liquid
  • Cotton woven fabric scouring method based on trametes and white-rot fungus combined fermentation crude enzyme liquid
  • Cotton woven fabric scouring method based on trametes and white-rot fungus combined fermentation crude enzyme liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]Embodiment 1: the preparation method of composite crude enzyme liquid

[0033] (1) Trametes CGMCCNo.10489 and White Pythium In order to produce strains, spores (the ratio of the number of spores is about 1:5) are directly inserted into the liquid seed medium, and cultured at 28°C and 150r / min for 36h.

[0034] (2) With 10% (v / v) inoculum amount, insert the seed liquid into the fermentation medium and cultivate for 5 days to ferment and produce enzyme under the condition of 28°C and 150r / min; after the fermentation is finished, the fermentation liquid is centrifuged at 4000rpm for 30min, and the supernatant is taken The enzyme activity is measured, and the fermentation supernatant is the compound crude enzyme liquid.

[0035] Enzyme activity was measured to obtain laccase activity of 3080.8U / L, cellulase activity of 191U / L, hemicellulase activity of 16271.8U / L, pectinase activity of 16800U / L, lignin peroxidase activity of 2000U / L L.

Embodiment 2

[0036] Embodiment 2: the preparation method of composite crude enzyme liquid

[0037] Trametes CGMCCNo.10489 and white rot The spores (ratio of the number of spores is about 5:1) were inserted into the liquid seed medium, cultivated at 25°C and 180r / min for 40 hours to obtain the seed liquid, and then the seed liquid was inserted into the fermentation culture according to the inoculation amount of 12% by volume Base, cultured at 25°C and 180r / min for 6 days to ferment for enzyme production, centrifuged after fermentation to get the supernatant, which was the compound crude enzyme solution.

[0038] Take the supernatant to measure the enzyme activity, laccase activity 3298.2U / L, cellulase activity 182U / L, hemicellulase activity 15971.8U / L, pectinase activity 15960U / L, lignin peroxidation Physical enzyme 1880U / L.

Embodiment 3

[0039] Embodiment 3: the preparation method of composite crude enzyme liquid

[0040] Trametes CGMCCNo.10489 and white rot The spores were inserted into the liquid seed medium, cultivated at 30°C and 120r / min for 32h to obtain the seed liquid, and then the seed liquid was inserted into the fermentation medium according to the inoculum amount of 8% by volume, and cultivated under the conditions of 30°C and 120r / min Under culture for 4 days to ferment for enzyme production, centrifuge after the end of fermentation to take the supernatant, which is the compound crude enzyme solution.

[0041] Take the supernatant to measure the enzyme activity, laccase activity 3302.1U / L, cellulase activity 183U / L, hemicellulase activity 15979.81U / L, pectinase activity 16422U / L, lignin peroxidation Physical enzyme 1928U / L.

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Abstract

The invention discloses a cotton woven fabric scouring method based on trametes and white-rot fungus combined fermentation crude enzyme liquid, and belongs to the technical field of textiles. According to the method, inhibition pectinase and laccase high-yielding strain trametes obtained through screening and commercially available lignin peroxidase high-yielding strain white pythium are subjected to combined fermentation culturing to produce crude enzyme liquid, and then the crude enzyme liquid is used for conducting cotton woven fabric scouring, wherein the laccase is one of ligninase, the preservation number of the trametes is CGMCC No.10489, the lignin peroxidase is one of the ligninase, and the preservation number of the white pythium is shown in the description. The compound crude enzyme liquid is a complex enzyme system containing the laccase, the lignin peroxidase, cellulase, hemicellulase, pectinase and the like, impurities of cellulose concomitants such as pectin, cotton wax and nitrogen substances, cotton seed hulls and the like on low-quality cotton fibers can be degraded, removing of the natural impurities on the low-quality cotton fibers through a one-bath-one-step method is achieved, and the technical obstacles of enzyme scouring industrial production of cotton woven fabrics are fundamentally solved.

Description

technical field [0001] The invention relates to a cotton fabric scouring method based on combined fermentation of Trametes and white rot fungi and belongs to the technical field of textiles. Background technique [0002] Pretreatment of cotton fabrics, including raw fabric preparation, singeing, desizing, scouring (scouring), bleaching, opening, rolling, drying and mercerizing processes to remove pectin, wax, cottonseed hulls from fibers and pulp and other impurities, improve the appearance and internal quality of the fabric. [0003] At present, the scouring of removing natural impurities on cotton fibers is currently using the chemical method of caustic soda treatment. The use of hot sodium hydroxide can saponify the fatty acid lipids in the waxy substances on the cotton fibers, and the fatty acids can form sodium salts, which can make the pectin substances And nitrogen-containing substances are hydrolyzed into soluble substances and removed. The cottonseed hulls will swe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M16/00D06M101/06
CPCD06M16/003D06M2101/06D06M2200/50
Inventor 王强范雪荣张颖王平袁久刚余圆圆崔莉
Owner JIANGNAN UNIV
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