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Composite membrane based on functional protein

A composite membrane and protein technology, which is applied in the field of functional protein composite membranes, can solve the problems of low functional protein content, low protein content, cumbersome process, etc., and achieve the effects of increasing the amount of embedding, simple method and process, and improving the degree of binding and stability.

Active Publication Date: 2016-04-20
YANTAI LVSHUIFU MEMBRANE MATERIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of phospholipid bilayer phospholipids or microcapsules self-assembled by phospholipids or amphiphilic copolymers as the supporting structure of functional proteins is not only costly but also tedious. The content of functional proteins that can be embedded in the assembled microcapsules is less, and the content of aquaporins introduced into the basal support layer will be even less, thus limiting the substantial increase in water flux of functional protein biomimetic separation membranes to a large extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0021] The polysulfone ultrafiltration basement membrane was placed in a dopamine Tris-HCl buffer solution with a concentration of 2 mg / mL, placed on a constant temperature water bath shaker at 30° C. for 6 hours, and the pH of the dopamine solution was 9.0. After the reaction, the surface of the membrane was rinsed three times with ethanol and deionized water. Then the dopamine-modified membrane was put into the PEITris-HCl buffer solution with a pH value of 9.0 to react for 6 hours, and the PEI concentration was 1g / L to prepare a positively charged polysulfone ultrafiltration membrane basement membrane. Soak the modified basement membrane in a polyanion electrolyte solution containing aquaporin Z and PSS. The concentration of the solution is 1g / L, the molar ratio of aquaporin Z and PSS is 0.01, and the soaking time is 30min. Clean the surface three times, soak again in the PEI polycation solution with a concentration of 1g / L for 30min, and wash the surface three times with d...

Embodiment example 2

[0023] The polyvinylidene fluoride ultrafiltration basement membrane was placed in a dopamine Tris-HCl buffer solution with a concentration of 2 mg / mL, and placed on a constant temperature water bath shaker at 30° C. for 6 hours. The pH of the dopamine solution was 9.0. After the reaction, the surface of the membrane was rinsed three times with ethanol and deionized water. Then the dopamine-modified membrane was put into PEITris-HCl buffer solution with a pH value of 9.0 to react for 6h, and the PEI concentration was 1g / L to prepare a positively charged polyvinyl fluoride ultrafiltration membrane basement membrane. Soak the modified basement membrane in a polyanion electrolyte solution containing aquaporin Z and PAA. The concentration of the solution is 2g / L, the molar ratio of aquaporin Z and PAA is 0.5, and the soaking time is 30min. Clean the surface three times, then soak the PEI polycation solution with a concentration of 2g / L for 30min, and wash the surface three times w...

Embodiment example 3

[0025]The polycarbonate basement membrane was put into a dopamine Tris-HCl buffer solution with a concentration of 2 mg / mL, and placed on a constant temperature water bath shaker at 30° C. for 6 h, and the pH value of the dopamine solution was 9.0. After the reaction, the surface of the membrane was rinsed three times with ethanol and deionized water. Then put the dopamine-modified membrane into the PEITris-HCl buffer solution with a pH value of 9.0 to react for 6h, and the PEI concentration was 1g / L to prepare a positively charged polyvinyl fluoride ultrafiltration membrane basement membrane. Soak the modified basement membrane in a polyanion electrolyte solution containing aquaporin Z and sodium alginate, the concentration of the solution is 1g / L, the molar ratio of aquaporin Z and sodium alginate is 10, and the soaking time is 30min. Wash the surface with deionized water three times, soak again in the PEI polycation solution with a concentration of 1g / L for 30min, and wash ...

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Abstract

The invention relates to a composite membrane based on a functional protein and a preparation method thereof. The composite membrane consists of a polyelectrolyte active layer on which the functional protein is embedded, and a supporting layer, wherein the functional protein is used as a functional unit and is directly embedded into a formed network structure in the process of assembling layer by layer to prepare the polyelectrolyte active layer, and by cross-linking and curing, combination firmness of the functional protein in the active layer is improved. The preparation method is simple in operation process, and embedding content of the functional protein in the composite membrane is improved. The functional protein composite membrane has a wide application prospect in the fields of water treatment and membrane separation.

Description

technical field [0001] The invention relates to the technical field of functional protein composite membranes, in particular to a composite membrane composed of a polyelectrolyte active layer containing functional proteins and a preparation method thereof, which can be used for seawater desalination, sewage treatment, dye removal, food processing, biomedicine and diseases fields of treatment. Background technique [0002] The extreme scarcity of fresh water resources, the rapid expansion of the energy gap and the increasingly serious environmental pollution are the important bottlenecks restricting the healthy and sustainable development of human civilization today. Membrane separation technology is widely used in sewage treatment, seawater desalination and other fields due to its advantages of high efficiency, energy saving and environmental protection. However, in the process of preparation and application of separation membranes, there is a "trade-off" effect between the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/74B01D69/10B01D67/00C08J7/12C08J7/04C08J3/24C08L81/06C08L27/16C08L69/00D06M15/356D06M15/15D06M13/123D06M101/28
CPCB01D67/0006B01D69/10B01D71/74B01D2323/30C08J3/24C08J7/042C08J7/12C08J2327/16C08J2369/00C08J2381/06C08J2405/04C08J2425/18C08J2433/02C08J2439/00C08J2479/02C08J2489/00D06M13/123D06M15/15D06M15/3562D06M15/3566D06M2101/28
Inventor 胡云霞刘中云戚龙斌
Owner YANTAI LVSHUIFU MEMBRANE MATERIAL
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