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Preparation for preventing and controlling pepper blight and application

A pepper fusarium wilt and preparation technology, applied in the application, pest control, biocide and other directions, can solve the problems of threatening the ecosystem, drug-resistant environmental pollution, affecting human health, etc., and achieve growth inhibition, ecological environment friendly, and plant enhancement. The effect of disease resistance

Inactive Publication Date: 2016-04-20
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional chemical agents, such as dexamethasone, pentachloronitrobenzene, carbendazim, etc., can achieve the purpose of preventing and controlling such diseases, but at the same time they also bring about a series of problems such as drug resistance and environmental pollution, which not only affect human healthy, and a serious threat to the entire ecosystem
The pathogenic bacteria of soil-borne diseases parasitize in the soil, and it is generally difficult to fundamentally control the harm of chemical pesticides

Method used

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  • Preparation for preventing and controlling pepper blight and application
  • Preparation for preventing and controlling pepper blight and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Acquisition, Identification and Preservation of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC

[0018] 1. Obtaining of Bacillus amyloliquefaciens YN2010-KC of the present invention

[0019] 1.1 Materials

[0020] The soil was collected from the soil around the corn rhizosphere of the Houshan Farm of Yunnan Agricultural University.

[0021] 1.2 Culture medium preparation

[0022] Melt LB solid medium and PSA medium (potato sucrose agar medium), pour them into sterilized petri dishes, cool down, and make LB and PSA plates.

[0023] 1.3 Isolation and culture

[0024] A strain isolated and purified was named YN2010-KC by using dilution plate separation method and plate streak separation.

[0025] 2. Identification of Bacillus amyloliquefaciens YN2010-KC

[0026] 2.1. Morphological identification

[0027] The bacterial strain YN2010-KC was cultured on LB medium, cultured at 37°C for 36 hours, and then observed and identified by morphologic...

Embodiment 2

[0032] Embodiment 2 The preparation method of the preparation for preventing and treating capsicum wilt of the present invention comprises the following steps:

[0033] (1) Preparation of primary seed solution

[0034] Bacillus amyloliquefaciens YN2010-KCCGMCC No.5722 stored at -80°C was inoculated on liquid LB medium and cultured at 28-37°C for 24-36 hours to obtain activated strains. Inoculate the activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 into the seed culture medium at a 10% inoculum amount, and cultivate it on a shaking table for 36 hours at a culture temperature of 35°C and a rotation speed of 120 rpm / min to obtain a first-grade Seed liquid, the seed medium is liquid LB medium, pH7.0~7.2;

[0035](2) Transfer the primary seed solution to the secondary seed medium in the seed tank at 35°C with a rotation speed of 100 rpm / min, an air flow of 1000 L / h, and a tank pressure of 0.02 to 0.03 at 10% inoculum size. Cultivate under MP...

Embodiment 3

[0038] Example 3 Antagonistic effect of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 of the present invention on various pathogenic bacteria of pepper

[0039] The antagonistic effect of Bacillus amyloliquefaciens YN2010-KCCGMCCNo.5722 on two pathogenic bacteria, Alternaria alternata and Fusarium oxysporum, was tested by plate confrontation culture method, and inserted in the center of a PSA plate with a diameter of 9 cm. The pathogenic fungus cake with a diameter of 5 mm was inoculated with Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 at a distance of 3 cm from the cake according to the cross position. Incubate the dish upside down at 25°C. After the diameter of the control colony reaches 9 cm, measure the width of the inhibition zone, measure the diameter of the treated colony by the cross method, and calculate the growth inhibition rate of the colony. The experiment was repeated 3 times. Bacillus amylolique...

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Abstract

The invention discloses a preparation for preventing and controlling pepper blight and application. The active ingredient of the preparation is fermentation liquid of individual-plant hydrolyzed starch Bacillus amyloliquefaciens YN2010-KC with CGMCC No.5722. The preparation has growth promoting and disease preventing effects simultaneously and can remarkably inhibit the growth of plant fungi, the growth inhibition ratio to the plant fungi, such as black sigatoka and oxysporum of peppers, is up to 62.44%-77.33%, and the prevention and control effect on the oxysporum of the peppers is up to 53.75%-68.54%. The preparation is a non-toxic, pollution-free and residue-free microbial product safe to people and livestock and friendly to an ecological environment.

Description

technical field [0001] The invention relates to the prevention and treatment of fusarium wilt of pepper, in particular to a preparation of bacillus amyloliquefaciens and its application for prevention and control of fusarium wilt of pepper. Background technique [0002] Pepper wilt, also known as pepper wilt, is caused by the infection of Fusarium Oxysporium (Fusarium Oxysporium), a kind of devastating world soil-borne plant disease. Vascular bundles in the rhizome of pepper, the diseased part turns brown, and finally causes the whole plant to wither and die. The disease has been reported abroad, and was first reported by Yu Dafu in my country in the 1950s. In recent years, it has commonly occurred all over the country, and has become one of the main diseases in greenhouse pepper cultivation, and it is becoming more and more serious. The incidence rate of the disease is generally 15% to 30%, and when it is severe, it reaches 70% to 80%. The fields withered and died, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02C12N1/20A01P3/00A01P21/00C12R1/07
CPCA01N63/10C12N1/20
Inventor 吴毅歆何月秋何鹏搏何鹏飞
Owner YUNNAN AGRICULTURAL UNIVERSITY
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