Application of Lysozyme Two-Dimensional Nanofilms as Antibacterial Materials

一种二维纳米、抗菌材料的技术，应用在应用、杀菌剂、用于生物控制的化学品等方向，能够解决难以形成连续的薄膜结构、稳定性不好、机械强度低等问题，达到广谱和高效抗菌性能、良好抗菌效率、操作简便的效果

Active Publication Date: 2020-06-26
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, as a water-soluble protein molecule, lysozyme has the common disadvantages of protein molecules, such as low mechanical strength, difficulty in forming a continuous film structure on a solid surface, poor stability, and rapid dissolution in aqueous solution, etc.
These characteristics limit its application to some extent

Method used

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  • Application of Lysozyme Two-Dimensional Nanofilms as Antibacterial Materials
  • Application of Lysozyme Two-Dimensional Nanofilms as Antibacterial Materials
  • Application of Lysozyme Two-Dimensional Nanofilms as Antibacterial Materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0025] Add 0.1433g of tris(2-carboxyethyl)phosphine to 10mL of 10mmol / L 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 7.4, adjust the pH value to 6.4 with NaOH, and prepare 50mmol / L 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of tris(2-carboxyethyl)phosphine; add 40mg lysozyme to 10mL 10mmol / L 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with pH value of 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of 4mg / mL lysozyme; take 10mL 50mmol / L tris (2-carboxyethyl) phosphine 4-hydroxyethylpiperazineethanesulfonic acid buffer solution Mix well with 10mL 4mg / mL lysozyme in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, let it stand at room temperature for 50 minutes, and form a layer of film on the surface of the mixture, that is, lysozyme two-dimensional nano film, and then wash with ...

Embodiment 2

[0027] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0028] Add 0.1433g of tris(2-carboxyethyl)phosphine into 10mL of 10mmol / L tris(2-carboxymethyl)aminomethane buffer solution with a pH value of 7.4, adjust the pH value to 6.4 with NaOH, and prepare 50mmol / L of tris(2- Carboxyethyl) phosphine tris buffer solution; 40mg lysozyme was added into 10mL 10mmol / L tris buffer solution with a pH value of 7.4 to prepare 4mg / mL trihydroxymethyl aminomethane buffer solution of lysozyme Methylaminomethane buffer solution; the tris (2-carboxyethyl)phosphine tris buffer solution of 10mL 50mmol / L is mixed with the tris buffer solution of lysozyme of 10mL4mg / mL, Insert the medical plastic catheter into the mixed liquid, let it stand at room temperature for 50 minutes, and directly form a layer of self-assembled film on the inner and outer surfaces of the medical plastic catheter (see figure 1 ), that is, a two-dimensional nanofilm of lyso...

Embodiment 3

[0030] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0031] The preparation method of lysozyme two-dimensional nano-film in the present embodiment is the same as embodiment 1, then uses agarose gel as medium to transfer lysozyme two-dimensional nano-film to the scalpel surface (see figure 2 ), play the role of antibacterial protection.

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Abstract

The invention discloses an application of a lysozyme two-dimensional nano-film as an antibacterial material. According to the application, the lysozyme two-dimensional nano-film serves as the antibacterial material, the lysozyme two-dimensional nano-film is formed on the surface of a substrate through in-situ growth only during the preparation of the lysozyme two-dimensional nano-film, or the prepared lysozyme two-dimensional nano-film is directly adhered to the surface of the substrate and can be formed on surfaces of substrates of various shapes through in-situ growth or adherence. Accordingto the application, the lysozyme two-dimensional nano-film serves as the antibacterial material and has broad-spectrum and efficient antibacterial performance, an antibacterial spectrum can be extended to three species of bacteria, i.e., gram-positive bacteria, gram-negative bacteria and fungi from category I bacteria of Classic gram-positive bacteria, the lysozyme two-dimensional nano-film presents good antibacterial efficiency to the three species of bacteria, and the antibacterial efficiency basically can reach 90% or more in 2 hours; the lysozyme two-dimensional nano-film has good adhesivity to substrates to be protected and is free of toxicity and adsorptivity to cells; and thus, the lysozyme two-dimensional nano-film has good antifouling properties to the cells and can serve as an ideal antibacterial food packaging material and a medical application program material.

Description

technical field [0001] The invention belongs to the technical field of antibacterial materials, and in particular relates to the application of a two-dimensional nano film of lysozyme as an antibacterial material. Background technique [0002] Imparting an antimicrobial property is very important in a variety of fields including packaging, textile industry, medicine, water purification and health care. Antibacterial composite materials are widely used due to their excellent comprehensive properties. This composite antibacterial material can assemble different types and functional substances according to different needs. These characteristics endow composite materials with more and newer functions. However, composite antibacterial materials also have their own disadvantages. First, the monomers used to prepare composite antibacterial materials have different chemical stability relative to the surrounding medium, which will lead to unstable structure of composite materials an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/46A01N25/34A01P1/00A01P3/00
CPCA01N25/34A01N63/00
Inventor 杨鹏古金
Owner SHAANXI NORMAL UNIV
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