A method for analyzing differentially expressed proteins associated with neuronal injury

A technology for differentially expressed proteins and neuron damage, which can be used in the analysis of materials, material analysis by electromagnetic means, material separation, etc., and can solve problems such as lack of drug treatment mechanism

Active Publication Date: 2019-08-27
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Abstract
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Problems solved by technology

In the research on the mechanism of Yangxue Qingnao Granules in the treatment of chronic cerebral insufficiency, there is a lack of research on the mechanism of drug treatment with protein expression as an indicator.

Method used

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  • A method for analyzing differentially expressed proteins associated with neuronal injury
  • A method for analyzing differentially expressed proteins associated with neuronal injury
  • A method for analyzing differentially expressed proteins associated with neuronal injury

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Embodiment Construction

[0136] The present invention is further illustrated by the following examples, but not as a limitation of the present invention.

[0137] Instruments and reagents

[0138] The ultra-speed low-temperature centrifuge is a product of Beckman; high-performance liquid chromatography: model: RIGOL3220 (Beijing Puyuan Jingdian); chromatographic column: C18 reversed-phase column (Agela, C18 chromatographic column, 250 × 4.6mm i.d., filler particle diameter: 5 μm); Tianjin Bona Aigel; Eksigent liquid phase-AB SCIEX TripleTOF 5600 mass spectrometer and tandem mass spectrometry data analysis using ProteinPilot 4.2 software are ABI company products.

[0139] iTRAQ Kit: iTRAQ Reagent Multi-Plex Kit (Applied Biosystems); Protein Concentration Detection Kit (Bradford Company); Tris (BBI Company); NaF (Fluka Company); Benzamidine (Benzamidine) and 4- (2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) was purchased from Sigma; Leupeptin and Aprotinin were purchased from Shanghai Bio...

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Abstract

The present invention relates to a method for analyzing differentially expressed proteins related to neuron damage. The method comprises the following steps: step 1, constructing and grouping zebrafish brain and central nervous system damage models; step 2, using drugs for the drug group Processing; step 3, extraction of total protein in each group; step 4, mass spectrometry loading analysis. Step 5, bioinformatics analysis.

Description

[0001] Technical field: [0002] The present invention relates to the use of a protein analysis method in screening drugs, in particular to an analysis method for screening a differentially expressed protein related to neuron damage for the treatment of chronic cerebral insufficiency [0003] Background technique: [0004] Yangxue Qingnao Granule is a new traditional Chinese medicine independently developed by Tasly for the treatment of chronic cerebral ischemia caused by blood deficiency and liver hyperactivity. Mainly by improving meningeal microcirculation, increasing cerebral blood flow, significantly reducing the symptoms of neurological deficits caused by cerebral ischemia, significantly inhibiting nerve cell apoptosis and synaptic structural changes, and playing a role in the prevention and treatment of pathological changes in chronic cerebral ischemia . Neuronal damage caused by ischemia-reperfusion injury is one of the main causes of chronic cerebral insufficiency, an...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N27/62
Inventor 佟玲李晓稳李东翔陈楠孙万阳周水平朱永宏
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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