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A strain of Acremonium for controlling Myzus persicae

A kind of technology of acremonium acremonium and bacterial strain, applied in the field of microbial control

Active Publication Date: 2018-12-07
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical control is still the most effective way to control Aphids, and there are also many successful applications of biological control methods (such as the reproduction and release of the natural enemy Aphis persicae, etc.), but the use of marine microbial secondary metabolites to explore The development of biological agents for aphids has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Field control effect experiment of embodiment 1 bacterial strain mfn1 fermentation supernatant to tobacco aphid

[0033]The strain mfn21 was fermented and cultivated in large quantities. After 8 days, the fermentation liquid was collected, the bacteria were filtered through 8 layers of gauze, and the fermentation supernatant was taken for field control effect experiments. During the peak period of tobacco aphid occurrence in the tobacco field, use a 1L sprayer to evenly spray the fermentation supernatant on the front and back of the leaves with aphids on the upper part of the tobacco plant. Investigate the base number of insect population before spraying, and investigate the number of remaining live insects on the 1st, 3rd, and 7d after spraying. According to the investigation results, the corrected control effects of the fermentation supernatant on the 1st, 3rd, and 7th days are 78.49% and 78.49%, respectively. 90.51%, 92.13%. The results showed that the fermentation ...

Embodiment 2

[0034] Example 2 Bioassay of strain mfn1 secondary metabolite active substance on aphid

[0035] Carry out large-scale fermentation and culture of the strain mfn1, collect the fermentation broth after 8 days, filter the bacteria through 8 layers of gauze, extract 3 times with an equal amount of ethyl acetate, combine the organic phase for distillation, and transfer the obtained substance to a 5 mL centrifuge tube for elution with 5 mL of acetone When the room temperature is evaporated until the liquid level no longer drops, it is used as a bioassay solution for later use. Select tobacco leaves with 10-15 aphids and place them in a petri dish. Dilute the solution to be tested with acetone into 5 concentration gradients, namely 500 μL / mL, 250 μL / mL, 125 μL / mL, 62.5 μL / mL, and 31.25 μL / mL. Use a 0.0776 μL capillary micro-dropper and use the drop method. Spot measurement, set acetone as a control, repeat 4 times for each treatment, investigate and count the number of dead aphids ...

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PUM

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Abstract

The object of the present invention is to provide a bacterial strain for preventing and treating aphids, that is, a strain of Acremonium sp. from marine bottom mud, and the preservation number is: CGMCC NO.11658. The mfn1 bacterial strain screened by the present invention is used to prevent and control aphids, and can also be used to prepare products with the function of preventing and controlling aphids. The mfn1 bacterial strain with biological activity on tobacco aphid obtained by the present invention can be applied to the prevention and treatment of tobacco aphid, and the biological measurement of aphid by micro-drop method shows that the lethality of secondary metabolites in its fermentation liquid to aphid Up to 97.78%. After chemical analysis and structural identification by GC / MS, the secondary metabolites included 7 main compounds, including 3 peptide compounds, 2 ester compounds, alkaloid accumulation compounds, and hydrazine compounds, 1 each.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to a bacterial strain for preventing and controlling aphids, that is, a strain of Acremonium sp. from marine bottom mud, and secondary small molecules obtained from the bacterial strain The mixture of compounds; the bacterial strain of the present invention and its metabolites can be used in the control of tobacco aphids. Background technique [0002] As people pay more and more attention to their own health and living environment, the disadvantages brought by traditional chemical pest control measures have attracted great attention. The excessive use of chemical pesticides has led to the increase of pesticide residues in the environment, the enhancement of pest resistance and the re-emergence of pests and other problems are the most prominent. With the further understanding of various social problems, environmental problems, and human health problems caused b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 王秀芳陈丹任广伟王凤龙徐蓬军王新伟
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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