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Anti-senile dementia activity of bryostatins and application thereof

A technology for senile dementia and bryozoa, applied in the field of medicine, can solve problems that have not been reported before

Inactive Publication Date: 2016-04-06
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is no report on the anti-senile dementia activity of total lactones of bryozoa at home and abroad

Method used

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  • Anti-senile dementia activity of bryostatins and application thereof
  • Anti-senile dementia activity of bryostatins and application thereof
  • Anti-senile dementia activity of bryostatins and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Extraction and purification of total lactones from Carex

[0036] Freshly collected Bugulaneritina Linnaeus raw material (10kg, dry weight) was extracted with 95% ethanol at room temperature for one week, and extracted 4 times (300L / time, 4 times). The ethanol extracts were combined and the ethanol was recovered under reduced pressure. Extract 320g. The extract was suspended and dispersed with 90% methanol, and extracted with n-hexane 5 times with 5 L each time to obtain 56 g of n-hexane extract, which was inactive after screening for in vitro anticancer activity. Add water to the aqueous methanol layer to make an 80% methanol aqueous solution, use CCl 4 Extraction (1L / time, 5 times) to get CCl 4 The extract 9g (IC50=7μg / mL, P388) is the active site. The active site was subjected to rapid silica gel column chromatography (200-300 mesh) to obtain the active component 2BH-10 (0.41g, IC50=2.4g / mL, P388). 2BH-10 was subjected to SephadexLH-20 gel column chromatogr...

Embodiment 2

[0037] Example 2. Experiment on the biological activity of Carex wormwood against Alzheimer's disease

[0038] 1 Experimental materials

[0039] 1.1 Experimental animals: ICR mice, male, 6-8 weeks old, 18-22g, Dalian Medical University, 1.2 Reagents and drugs: Acetylcholinesterase (AChE) kit (Nanjing Jiancheng Institute of Biological Engineering), acetylcholine transferase ( ChAT) Kit (Nanjing Jiancheng Institute of Biological Engineering), Glutathione Peroxidase Test Kit (Nanjing Jiancheng Institute of Biological Engineering), Trace Reduced Glutathione Kit (Nanjing Jiancheng Institute of Biological Engineering), Acetylcholine kit (Nanjing Jiancheng Institute of Biological Engineering), SOD kit (Nanjing Jiancheng Institute of Biological Engineering), Malondialdehyde Test Kit (Nanjing Jiancheng Institute of Biological Engineering), Vitamin E (Dalian Tianyu Aosen Pharmaceutical Co., Ltd.), Test drug: Bryophyllum total lactones (BRYS) obtained in Example 1.

[0040] 1.3 Instruments: m...

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Abstract

The invention relates to the technical field of medicine, in particular to the anti-senile dementia activity of bryostatins and application thereof. According to the bryostatins (BRYS), mouse water maze experiments are conducted, the swimming time can be significantly shortened, obvious improvement effects on acetylcholine esterase (AChE) and acetylcholine (Ach) in mouse brains are achieved, the contents of choline acetyl transferase (ChAT) and malondialdehyde (MDA) are both obviously increased, the effect is significant, and differences are statistically significant. The SOD activity in serum can be significantly increased, P is smaller than 0.01, and the levels of serum reduced glutathione (GSH) and serum glutathione peroxidase (GSH-PX) are increased. In addition, the bryostatins has no obvious influence on the quality of mice and visceral indexes, and it is shown that the toxicity is low. It is shown through experimental results that the bryostatins has significant anti-senile dementia activity and lower toxicity and can be applied to development of drugs for resisting senile dementia.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to the anti-senile dementia activity of the total lactones of Bryophyllum spp. and its application in the medical field. Background technique [0002] Marine bryozoans are commonly known as bryozoans, sea mats, fake corals, and bryozoans. They are euphora and are one of the important components of marine benthic animals. There are many kinds of them, about 4,000 kinds, the most common one is the bryophyllum. Bugulaneritina Linnaeus is widely distributed in the waters of the world, and it is found in the salty waters of the Xisha Islands in the coast of my country from the Bohai Sea to the South China Sea. The colony is reddish brown or purple-brown, grass-like, 40-150mm high, each with two rows of insects, arranged alternately. Its colonies are often attached to kelp, agar, shellfish, ship bottoms, docks, navigation marks and other underwater facilities, affecting marine aquaculture, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/655A61P25/28A23L33/10A23L17/00
CPCA23V2002/00A23V2200/322A23V2250/2042
Inventor 易杨华
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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