Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell model for diabetes complicated with depression, and establishing method and application thereof

A technology for establishing methods and cell models, which is applied in the biological field and can solve problems that have not been reported yet

Inactive Publication Date: 2016-03-30
HUNAN UNIV OF CHINESE MEDICINE
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Regarding the cell model of depression and the cell model of diabetes complicated with depression, no one has reported or applied for a related invention patent so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell model for diabetes complicated with depression, and establishing method and application thereof
  • Cell model for diabetes complicated with depression, and establishing method and application thereof
  • Cell model for diabetes complicated with depression, and establishing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 In vitro culture of hippocampal neuron primary cells

[0028] The day before the experiment, the cell culture plate was pre-coated with 40mg / ml L-polylysine. On the day of the experiment, the polylysine in the culture plate was aspirated, and gently washed with PBS 3 times to clean the natural environment in the workbench. Let dry and set aside. The 18-day-old SD rat was anesthetized with 10% chloral hydrate, and the fetus was quickly taken out and placed in a large petri dish, fully sterilized with 75% ethanol, and the whole brain was quickly taken out with ophthalmic scissors and placed in a small container containing pre-cooled D-Hanks. Clean the petri dish thoroughly, and then use curved forceps to carefully lift the surface of the cerebral cortex to the outside under a stereomicroscope, and you can see a clear "bow-shaped" hippocampus, one on the left and one on the left, carefully clamp the hippocampus and separate it from the marginal tissue . Put ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biotechnology and especially relates to a method for establishing a cell model for diabetes complicated with depression. The method comprises the following steps: adding glucose with a final concentration of 50 to 200 mmol / L and corticosterone with a concentration of 100 to 400 [mu]mol / L into a medium for culturing hippocampal neurons and carrying out intervention culture on the hippocampal neurons for 18 to 24 h so as to obtain the cell model. The cell model is evaluated through general morphological observation, HE dyeing, glucose consumption detection, intracellular neurotransmitter detection, MTT-process detection, flow cytometry, Hoechst fluorescent staining and TUNEL staining; and evaluation results show that the cell model has good effect and can be used for pathological and physiological research on diabetes complicated with depression, research on pathogenesis, in-vitro drug screening or drug evaluation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cell model of diabetes complicated with depression and its establishment method and application. Background technique [0002] Diabetes is a common chronic metabolic disease with many complications. According to the World Health Organization survey in 2013, there are about 347 million diabetic patients in the world, and the incidence of depression in diabetic patients is 8.5% to 71.4%. Diabetes mellitus with depression (DD) patients have a suicide risk rate as high as 10%, which is much higher than the 10-30 / 100,000 people in the general population. Therefore, diabetes mellitus with depression has attracted more and more attention , and began to become one of the diseases that seriously endanger human health. [0003] However, there is no in vitro cell model that can simulate the clinical symptoms and signs of human DD, which greatly limits the pathophysiological resea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0793C12Q1/02
Inventor 王宇红刘检韩远山徐雅岚
Owner HUNAN UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com