A dispersant for two-phase fermentation system

A technology of dispersant and chelating agent, applied in the field of two-phase fermentation, can solve the problems of easy agglomeration, low conversion efficiency and yield, etc., and achieves the effect of solving agglomeration, reducing stirring and dispersing time, and having strong practicability.

Active Publication Date: 2019-06-11
SHANGHAI YUKING WATER SOLUBLE MATERIAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The object of the present invention is to provide a kind of dispersant for two-phase fermentation system, to solve the problem that the existing two-phase fermentation system substrate and organic phase fermentation process are easy to reunite, conversion efficiency and yield are not high, by adding dispersant agent to achieve the dispersion stability of substrates and organic matter, effectively improving the conversion efficiency and yield

Method used

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  • A dispersant for two-phase fermentation system
  • A dispersant for two-phase fermentation system
  • A dispersant for two-phase fermentation system

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Take 35g of methyl glucoside myristate, 40g of propyl alkyl carboxylate, 10g of fatty alcohol polyoxyethylene ether AEO-9, 5g of propylene glycol, 1g of EDTA-2Na, and 909g of water for subsequent use;

[0034] (2) Add methyl glucoside myristate, propyl alkyl carboxylate, AEO-9, and propylene glycol into a high-speed shearing machine and shear at a high speed to obtain a microemulsion with a particle size of about 300nm. Dissolve EDTA-2Na in water, dissolve evenly, add into the microemulsion, stir at 550r / min at high speed for 35min, and raise the temperature to 45°C at the same time; then stir at 100r / min at low speed for 30min, cool and filter to obtain the dispersant microemulsion of the present invention.

Embodiment 2

[0036] (1) Take 45g of sucrose ester SE-10, 35g of alkyl carboxylate heptyl ester, 15g of alkyl glycoside APG0810, 10g of butanediol, 5g of N-oleoyl sarcosine, and 890g of water for later use;

[0037] (2) Add sucrose ester SE-10, heptyl alkyl carboxylate, alkyl glycoside APG0810, and butanediol into a high-speed shearing machine and shear evenly at high speed to obtain a microemulsion with a particle size of about 250 nm. Dissolve N-oleoyl sarcosine in water, dissolve evenly, add it into the microemulsion, stir at 500r / min at high speed for 30min, and at the same time raise the temperature to 50°C; then stir at 120r / min at low speed for 30min, cool and filter to obtain the dispersion of the present invention agent microemulsion.

Embodiment 3

[0039] (1) Get 55g of sucrose ester SE-13, 25g of methyl phenol carboxylate, 5g of sorbitan ester-60, 15g of glycerol, 3g of EDTA-2Na, and 897g of water for subsequent use;

[0040](2) Add sucrose ester SE-13, methyl alkylphenol carboxylate, sorbitan ester-60, and glycerol into a high-speed shearing machine and shear at a high speed to obtain a microemulsion with a particle size of about 450 nm. Dissolve EDTA-2Na in water, dissolve evenly, add into the microemulsion, stir at 600r / min at high speed for 40min, and at the same time raise the temperature to 50°C; then stir at 150r / min at low speed for 30min, cool and filter to obtain the dispersant microemulsion of the present invention.

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Abstract

The invention discloses a dispersing agent for a two-phase fermenting system. The dispersing agent is prepared from, by weight, 3.5-5.5% of glucoside ester and derivatives of glucoside ester, 2.5-4.0% of long chain carboxylic acid ester, 1.5-5% of non-ionic surface active agent, 1.5-5% of wetting agent and 0.1-0.5% of chelating agent. The dispersing agent has the advantages that substrate organisms in the two-phase fermenting system can be rapidly dispersed into particles, ester substances are adsorbed to the peripheries of the particles to form a micelle-similar wrapping system so as to serve as dual-charge layers for keeping the dispersion of particles, the problem that a substrate and an organic phase of the two-phase fermenting system are prone to agglomeration in the fermenting process is effectively solved, and therefore the feed amount and conversion rate of the substrate are greatly increased, and yield is improved.

Description

technical field [0001] The invention belongs to the technical field of two-phase fermentation, and in particular relates to a dispersant used in a two-phase fermentation system. Background technique [0002] The development of two-phase fermentation systems was initially inspired by a series of problems encountered in enzyme-catalyzed reactions. It is generally believed that enzymes are only active in aqueous solution, and enzyme-catalyzed reactions are usually carried out in aqueous solution. However, at the end of the 19th century, with the development of the biocatalysis industry, some important compounds were found to be unstable in water or in the process of enzyme catalysis. If it is insoluble in water, it can only increase its solubility and speed up the reaction rate or even reduce its hydrolysis if it is carried out in an organic solvent. Therefore, some scholars proposed to use the two-phase system to solve this problem, and achieved success, laying a solid founda...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00
CPCC12P33/00
Inventor 王宇朱广东陈占熊俊超
Owner SHANGHAI YUKING WATER SOLUBLE MATERIAL TECH
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