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Microalgae solid-phase attached growth culture method with water and nutrition supplied based on capillary power

A cultivation method and capillary power technology, applied in the field of production and harvest of microalgae, can solve problems such as inability to save, and achieve the effect of saving resources and energy, saving energy, saving water resources and nitrogen and phosphorus nutrient sources

Active Publication Date: 2016-03-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the existing life cycle assessment, in the process of using microalgae to produce biodiesel, an average of 3,726kg of fresh water is needed to produce 1kg of biodiesel. Although the water recovery in the process can save most of the water required in the harvesting process, However, the demand for fresh water in the cultivation stage is a rigid requirement in traditional liquid phase cultivation, which cannot be saved.

Method used

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  • Microalgae solid-phase attached growth culture method with water and nutrition supplied based on capillary power
  • Microalgae solid-phase attached growth culture method with water and nutrition supplied based on capillary power
  • Microalgae solid-phase attached growth culture method with water and nutrition supplied based on capillary power

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preparation example Construction

[0029] The preparation of solid phase carrier: by comparing the performance of multiple materials, select the microfiber (such as figure 1 shown), placed flat in a laboratory plexiglass algae box. Use 5cm polyester 300D superfine fiber as a capillary drainage tube, one end is connected to the BG11 liquid medium, and the other end is in contact with the solid phase carrier. For large-scale production and application, the large-area solid-phase carrier needs to be divided into several solid-phase carriers with appropriate areas, and each solid-phase carrier needs to be introduced into the BG11 liquid medium through a capillary drainage tube.

[0030] Cultivation stage: the solid phase carrier inoculated with microalgae is cultivated under light conditions to the harvestable stage. Harvesting time depends on the purpose of cultivation. If the oil in algae cells is extracted, it should be harvested in the middle of the stable period.

[0031] Harvesting stage: The carrier with ...

Embodiment 1

[0032] Embodiment 1: small-scale intermittent microalgae cultivation method

[0033] Evenly inoculate Scenedesmus.LX10.267g / m on a 3cm×5cm microfiber carrier 2 . Pour 30ml of the prepared BG11 liquid medium into each glass petri dish, use 5cm superfine fiber bundles to connect the solid phase carrier and BG11 liquid medium, after 10-20 minutes, the BG11 liquid medium can pass through capillary action Damp the entire algae blanket. Place the algae blanket and medium in an artificial climate incubator with a temperature of 25±2°C, a humidity of 30%, a light intensity of 1100 lux, and a light-to-dark ratio of 14:10. During the cultivation period, ensure that there is always remaining medium in the glass dish by intermittently adding medium. At the end of the cultivation, the water used for each algae blanket is between 215mL-235mL. In addition, in order to ensure that the algae blanket is evenly illuminated, the front and back of the algae blanket are reversed once a day. Af...

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Abstract

The invention discloses a microalgae solid-phase attached growth culture method with water and nutrition supplied based on capillary power, and belongs to the technical fields of production and harvesting of microalgae. The culture method comprises the following steps: inoculating an algae solution on a solid-phase vector, and keeping the solid-phase vector on a transparent supporting system; and then on the basis of the capillary performance of the solid-phase vector, automatically sucking a BG11 liquid culture medium and storing in the solid-phase vector, and supplying water and nutrition to microalgae on the solid-phase vector, so that the growth of the microalgae is achieved, wherein the microalgae is scenedesmus, chlorella or selenastrum capricornutum. The culture method disclosed by the invention, when used for culturing the microalgae, can simplify culture and harvesting processes and save energy; and the culture method is applied to the industrial production of energy microalgae, edible fungi, microalgae bio-products and like products.

Description

technical field [0001] The invention belongs to the technical field of production and harvesting of microalgae, and in particular relates to a microalgae solid-phase attachment growth culture method based on capillary power for water supply and nutrition. Background technique [0002] Some microalgal cells contain a high proportion of oil, which can produce biodiesel through lipid conversion, which can be used as a renewable energy to replace traditional non-renewable energy such as coal and oil, and alleviate the global energy crisis. At the same time, in the process of large-scale production of microalgae, microalgae absorb atmospheric CO 2 , thereby reducing the amount of greenhouse gases in the atmosphere. Some microalgal cells, such as spirulina, have high food value because of their high protein and nutrient content. In addition, some microalgal cells, such as Haematococcus pluvialis, can synthesize astaxanthin in the stable phase, which has medicinal value. Realizi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 胡洪营许雪乔庄林岚巫寅虎童心
Owner TSINGHUA UNIV
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