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Method for preparing phenolic acid compound through fatsia japonica

An octagonal gold plate and phenolic acid technology are applied in the field of biotransformation of natural products to achieve the effects of large biomass, broad application prospects and wide planting area.

Inactive Publication Date: 2016-03-09
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, there is no research report on phenolic acid compounds in star anise gold plate

Method used

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  • Method for preparing phenolic acid compound through fatsia japonica
  • Method for preparing phenolic acid compound through fatsia japonica
  • Method for preparing phenolic acid compound through fatsia japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 500g of star anise golden pan fruit was crushed through a 60-mesh sieve, and 70% ethanol solution was added according to the ratio of solid to liquid at 1:20, ultrasonically extracted, and rotary evaporated until ethanol-free. The extract was extracted with ethyl acetate to obtain an extract containing 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and quinic acid And raffinate containing chlorogenic acid, caffeic acid, quinic acid. After the organic solvent of the extract was removed, further separation and purification by column chromatography obtained 0.28 g of 3,4-dicaffeoylquinic acid with a purity of 85%, and 2.9 g of 3,5-dicaffeoylquinic acid with a purity of 92%. 0.23g of 90% 4,5-dicaffeoylquinic acid, caffeic acid, quinic acid and other products can be obtained from the raffinate.

Embodiment 2

[0044] Same as in Example 1, the ethanol solution with a volume fraction of 70% was replaced with a methanol solution with a volume fraction of 70%.

Embodiment 3

[0046] 500g of star anise golden pan fruit was crushed through a 60-mesh sieve, and 70% ethanol solution was added according to the ratio of solid to liquid 1:20, ultrasonically extracted for 1 hour, and the operation was repeated once. The extract was extracted in equal proportions with ethyl acetate to obtain 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and quinic acid Extract and raffinate containing chlorogenic acid, caffeic acid, quinic acid. After the organic solvent of the extract was removed, the AB-8 macroporous resin was further separated and purified to obtain 0.31 g of 3,4-dicaffeoylquinic acid with a purity of 90%, and 2.85 g of 3,5-dicaffeoylquinic acid with a purity of 96%. g, 0.37 g of 4,5-dicaffeoylquinic acid with a purity of 90%, caffeic acid, quinic acid and other products can also be obtained from the raffinate.

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Abstract

The invention relates to a method for preparing a phenolic acid compound through fatsia japonica. According to the method, the phenolic acid compound is obtained with fatsia japonica as a raw material through extraction and separation or obtained through biological conversion of aspergillus niger and eurotium after extraction and separation. According to the method, the phenolic acid compound in fatsia japonica is researched and quantitatively analyzed for the first time, and the total amount of the phenolic acid compound in fatsia japonica is 0.279-1.38%. Besides, the yield of a dicaffeoylquinic acid compound is increased through the biological conversion method, and a new raw material and a new method are provided for obtaining the phenolic acid compound.

Description

technical field [0001] The invention provides a new source of raw materials and a preparation method for preparing phenolic acid compounds, especially a method for improving product production by using Aspergillus niger enzyme and Aspergillus solani enzyme liquid conversion, which belongs to the field of biotransformation of natural products. Background technique [0002] Phenolic acid compounds are a class of secondary metabolites widely present in plants derived from carbohydrate metabolism. They have a benzene nucleus and are mostly derivatives of p-hydroxybenzoic acid and p-hydroxybenzoic acid (cinnamic acid). There are mainly hydroxycinnamic acids, including caffeic acid, P-coumaric acid and ferulic acid, etc.; hydroxybenzoic acids, including protocatechuic acid and gallic acid, etc.; depsipolic acids, including chlorogenic acid and isochlorogenic acid Acid, etc. (Zhang Nan, Du Lili, Wang Dong, Liu Xiaoqiu. Research progress on phenolic acids in traditional Chinese medi...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/62C07C51/42C07C59/52C07C67/48C07C69/732C12R1/685C12R1/645
CPCC12P7/62C12P39/00C07C51/42C07C67/48C07C59/52C07C69/732
Inventor 陈育如李东晓宋婷婷
Owner NANJING NORMAL UNIVERSITY
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