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Method for extracting water soluble beta-glucan from sparassis crispa sporophore

A glucan, water-soluble technology, applied in the field of active ingredient extraction of traditional Chinese medicine, can solve the problems of poor polysaccharide absorption, difficult extraction, poor water solubility and the like

Inactive Publication Date: 2016-03-09
HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large molecular weight part of hydrangea polysaccharides has poor water solubility, and it is difficult to extract completely with water as a solvent in the separation and extraction. Even if the lye is used for extraction, although the extraction rate is high, the polysaccharide product obtained after drying is still insoluble in water when applied.
The human body does not absorb this macromolecular hydrangea polysaccharide well

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting water-soluble β-glucan from hydrangea fruiting body, characterized in that: comprising the following steps:

[0032] Step 1, prepare hydrangea crude polysaccharide:

[0033] Take Hydrangea sporocarp, pulverize to obtain powder, extract the powder with lye, filter to remove residue, add ethanol to the filtrate, let stand, filter to obtain precipitate, and obtain Hydrangea crude polysaccharide;

[0034] Step 2. Preparation of Hydrangea β-glucan:

[0035] Take the hydrangea crude polysaccharide, add water and stir after drying, add α-amylase to hydrolyze, filter the hydrolyzate, discard the filtrate, obtain the residue, and obtain Hydrangea β-glucan;

[0036] Step 3. Preparation of water-soluble β-glucan:

[0037] Take Hydrangea β-glucan, add lye to stir and extract, adjust the pH of the extract to pH 2.0-6.8, heat and hydrolyze, filter the hydrolyzate, add ethanol to the filtrate, let stand, separate the precipitate and dry to obtai...

Embodiment 2

[0045] Weigh 1 kg of Hydrangea sporocarp and pulverize it, pass through a 50-mesh sieve, add 5 liters of 0.1 mol / liter sodium hydroxide solution to the powder and extract for 1 hour, filter through a plate basket with a filter cloth of 0.5 micron pore size to obtain 4.5 liters of filtrate. Add 18 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket with a pore size of 0.5 micron to obtain a precipitate, which weighs a total of 150 grams after drying. Add 750 ml of water to the precipitate and stir at room temperature for 10 minutes, add 1 gram of fungal α-amylase solution (Shandong Jienuo Bioenzyme Co., Ltd.), adjust the pH value to 5.5 with 6 mol / L hydrochloric acid, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket with 0.5 micron aperture cloth, discard the filtrate, add 0.8 liter of 1.0 mol / L sodium hydroxide to the residue and stir for extraction for 2 hours, adjust the acidity and alkalini...

Embodiment 3

[0047] Weigh 1 kg of Hydrangea sporocarp and pulverize it, pass through a 50-mesh sieve, add 10 liters of 1 mol / liter sodium hydroxide solution to the powder and extract for 5 hours, filter through a plate basket with a 0.5-micron pore filter cloth to obtain 8.2 liters of filtrate. Add 32.8 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket of 0.5 micron aperture cloth to obtain a precipitate, which weighs a total of 142 grams after drying. Add 1.4 liters of water to the precipitate and stir at room temperature for 10 minutes, add 0.6 g of fungal α-amylase solution (purchased from Wuxi Chengxing Biotechnology Co., Ltd.), adjust the pH value to 5.8 with 2 mol / L hydrochloric acid, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket with 0.5 micron aperture cloth, discard the filtrate, add 0.8 liter of 1.0 mol / liter of sodium hydroxide to the residue and stir for extraction for 2 hours, adjust the ...

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Abstract

The present invention discloses a method for extracting water soluble beta-glucan from sparassis crispa sporophore, the water soluble beta-glucan is prepared by the steps of sparassis crispa crude polysaccharide preparation, sparassis crispa beta-glucan preparation, water soluble beta-glucan preparation, and the like, sparassis crispa sporophore polysaccharide prepared by the method is beta-glucan with alpha-configuration being removed, and the beta-glucan has good water solubility. Infrared spectroscopy shows that the product is the beta-glucan, phenol-sulfuric method detects that the polysaccharide content is greater than 50%, and the polysaccharide average molecular weight is 2000-200000 Dalton in variety. In the prior art, a particular configuration of sparassis crispa is separated and further purified to obtain high purity polysaccharide, but the separation process is essentially different from the method, according to the method, by acid hydrolysis and water re-dissolution method, the molecular weight of the sparassis crispa polysaccharide is controlled within a certain range for enhancement of the water-solubility of the sparassis crispa polysaccharide, the yield of high molecular weight segment sparassis crispa polysaccharide is improved, and industrial production of sparassis crispa beta-water-soluble polysaccharide is facilitated.

Description

technical field [0001] The invention relates to a method for extracting water-soluble β-glucan from Hydrangea sporophore and its product. The invention belongs to the field of active ingredient extraction of traditional Chinese medicine. Background technique [0002] Hydrangea, also known as hydrangea mushroom, the Latin scientific name is Sparassiscrispa, belongs to the order of non-folded fungi, hydrangea family, hydrangea genus. The biggest feature of hydrangea is that it contains a large amount of β-glucan. According to the analysis of the Japan Food Analysis Center, every 100g of Hydrangea contains 43.6g of β-glucan, which is 3~4 times higher than Ganoderma lucidum and Agaricus blazei. It can be said that hydrangea contains the most β-glucan in mushrooms. Glucan is a polysaccharide formed by the polymerization of glucose monomers. It is divided into α-type and β-type. α-type glucan, such as starch, is the main source of energy for the body and has no biological activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0024
Inventor 陈喜军
Owner HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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