An endophytic bacterium gj-06 of the mustard root nodule, a clubroot control agent containing the endophytic bacterium, and its application

Abstract

The invention discloses tumorous stem mustard root nodule ndophytic bacteria GJ-06, a clubroot preventing and treating agent containing same and application. The tumorous stem mustard root nodule ndophytic bacteria (Bacillus subtilis) GJ-06 is deposited the China Center for Type Culture Collection with accession number of CCTCC NO: M 2015665 on November 9, 2015; the clubroot preventing and treating agent comprises the tumorous stem mustard root nodule ndophytic bacteria GJ-06 which serve as effective ingredients; and the tumorous stem mustard root nodule ndophytic bacteria GJ-06 and the clubroot preventing and treating agent can be used for preventing and treating clubroot. By the ndophytic bacteria GJ-06, the clubroot index of tumorous stem mustard is reduced to 36.1 from 75.3, the preventing and treating rate on the tumorous stem mustard reaches 52%, field clubroot degree can be reduced effectively, and an unexpected effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of endophytes, and in particular relates to a kind of endophytic bacteria ( Bacillus subtilis ) GJ-06, a clubroot control agent containing the endophytic bacteria and its application. Background technique [0002] Plasmodium brassicae ( Plasmodiop hora brassicae Woron) belongs to the Plasmobacteriaceae of the protozoan kingdom Pluzobacteria Pluzobacteria Pluzobacteria Pluzobacteriaceae, which mainly harms cruciferous plants such as Chinese cabbage, rapeseed, mustard mustard, radish, and causes plant roots to swell, affecting plant absorption. The infection of clubroot causes the epidermis of the plant root to rupture, and other pathogens in the soil invade, causing the root system to rot, the plant to be short and yellow, or even die, and the yield to be severely reduced. Plasmodium brassicae can survive in the soil for a long time. Once the disease occurs in the field, it is difficult to eliminate, and...

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Problems solved by technology

Since the discovery of mustard clubroot in Fuling in 1994, the disease has become the main disease of mustard at present, with an incidence of 30-50% in the field. It is difficult to continue planting mustard in diseased fields, resulting in a decline in the planting area of ​​mustard, which seriously threatens mustard. The sustainable development of (Gao Mingquan, Peng Hongjiang, Wang Xu, et al. Determination of damage and yield loss of mustard clubroot in Fuling. Plant Protection, 2002, 28(6): 31-33; Xiao Chonggang, Guo Xianghua, Han Haibo, etc. Identification and main characteristics of clubroot pathogen of mustard mustard in Fuling. Journal of Southwest Agricultural University, 2002,24(4): 539-541)

The continuous cropping period of mustard mustard in Fuling area is 10-20 years. Long-term continuous cropping of a single crop will lead to continuous cropping obstacles, resulting in soil acidification, soil microbial ecosystem imbalance, and increased soil-borne diseases (. Zhang X, Pan Z, Zhou X, et al . Autotoxicity and Continuous Cropping Obstacles: A Review. Chinese Journal of Soil Science, 2007, 4: 33-38)

At present, the soil in the mustard continuous cropping field in Fuling area has been acidified, and the dormant spores of Plasmodium root endophytic fungus are more likely to survive in an acidic environment (Narisawak, Ohhi K T, Hashiba T. Suppressio n of clubroot and verticilli um yellows in Chinese cabbage in the field by the root endophytic fungus Heteroconi umchaetospir a . Plant Pathology, 2000, 49: 141-146), which also leads to the increase of clubroot year by year

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