A method for creating germplasm by distant compound hybridization of pickled mustard and radish
A technology of compound hybridization and pickled mustard, which is applied in the field of biological genetics, can solve the problems of large occurrence area, high incidence frequency, and difficulty in prevention and control, and achieve the effects of promoting replacement, less incidence, and solving poor stress resistance
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Embodiment 1
[0031] A method for creating germplasm by distant compound hybridization of pickled mustard and radish, the operation steps of which are as follows:
[0032] (1) Hybridization:
[0033] A, the selection of parents: choose well-developed, strong, no pests and diseases early-maturing mustard variety Linshicaoyaozi, mid-late-maturing varieties Suanye and late-maturing varieties Yubo, plants in the blooming period, as the female parent, choose well-developed, robust, non-toxic The radish with good resistance to diseases and insect pests is used as the male parent;
[0034] a) Treat the female parent: ① Arranging the inflorescence: remove the buds that have already bloomed, the yellow buds that are about to bloom, and the buds that are too small at the top, and only keep 20-25 buds in the middle, and then use tweezers to remove the buds that develop in the middle Bad flower buds; ②Detasselling: clamp the whole inflorescence between the thumb and middle finger of the left hand, wit...
Embodiment 2
[0047] The difference from Example 1 is only that: in step (1), the selected variety of mustard mustard female parent is: mid-ripening variety Yongan Xiaoye; Linshi grass kidney, all the other operations are consistent with embodiment 1.
Embodiment 3
[0049] The only difference from Example 1 is that the selection of mustard mustard female parent in step (1) is: mid-ripening mustard variety Pixian mustard; in step (4), other mustard varieties backcrossed are: semi-broken leaves, Yongan small leaves and Linshi grass kidney, all the other operations are consistent with embodiment 1.
[0050] It can be seen from this: by crossing a mustard variety with strong combining ability with radish to obtain distant hybrid offspring material, the genetic material of radish is introduced into mustard variety, and then the distant hybrid offspring material is used as an intermediate bridge to carry out distant hybridization. A large number of new germplasm materials of different mustard varieties and radish were obtained by compound crossing. At the same time, this intermediate bridge can be further hybridized with radish, and then hybridized with mustard to introduce the good genes of radish into mustard. And at the same time, this inte...
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