Copper ions detection method based on nano-cluster

A copper ion detection and nano-cluster technology, applied in the field of copper ion detection, can solve problems such as expensive instruments, and achieve the effects of high sensitivity, small detection interference, and rapid detection of copper ion concentration

Active Publication Date: 2016-02-24
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Description
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  • Application Information

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Problems solved by technology

[0003] At present, copper detection methods mainly include atomic absorption, inductively coupled plasma mass spectrometry, and atomic fluorescence spectroscopy, etc., which have the disadvantage of expensive instruments

Method used

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  • Copper ions detection method based on nano-cluster
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Embodiment Construction

[0025] The present invention will be further described below in conjunction with accompanying drawing:

[0026] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0027] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified. Chlorauric acid was purchased from Alfa

[0028] The water mentioned in the article (conductivity 18.2MΩ) is ultrapure water.

[0029] A method for detecting copper ions based on nano clusters, comprising the steps of:

[0030] 1) Mix the standard copper ion solution and the gold nanocluster solution to establish a standard curve between the fluorescence signal quenching of the detection nanocluster and the amount of copper ions; the serial concentration range of the standard copper ion solution is: 0.02-30 μM.

[0031] 2) Mix the sample solution and the gold nanocluster solution, and bring the fluorescence signal quenching ...

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Abstract

The invention relates to a copper ions detection method based on nano-cluster. The method is characterized by comprising the following steps: 1) mixing a standard copper ions solution and gold nanoclusters, establishing a standard curve of fluorescence signal quenching strength for detecting the nano-cluster and copper ions amount; and 2) mixing a sample solution and the gold nanoclusters, bring the fluorescence signal quenching strength for detecting the nano-cluster into the standard curve to obtain the content of copper ions in the sample. The concentration of the gold nanoclusters is 0.5 mM, a pH value is 7; and the gold nanoclusters are coated and modified by GSH. The experiment proves that the sensitivity of the detection method is high, detection interference of other ions to the copper ions is less, the method can realize selectivity detection to the copper ions, so that a purpose for rapidly detecting the concentration of copper ions can be reached, and the method has wide application prospect for copper ion detection.

Description

technical field [0001] The invention relates to the detection of copper ions, in particular to a method for detecting copper ions based on nano-clusters, which can realize the purpose of rapidly detecting the concentration of copper ions. Background technique [0002] Copper is a kind of heavy metal. Excessive copper ions will cause great harm to the human body, destroy protein activity, and cause tissue necrosis. Liver and gallbladder, so the detection of copper ions is of great significance to the environment and human health. [0003] At present, copper detection methods mainly include atomic absorption, inductively coupled plasma mass spectrometry, and atomic fluorescence spectroscopy, etc., which have the disadvantage of expensive instruments. [0004] Therefore, it is very meaningful to develop efficient, cheap, convenient and fast copper detection technology. Contents of the invention [0005] The purpose of the present invention is to provide a copper ion detecti...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 刘萍萍周会娜陈千思翟妞金立锋郑庆霞陈霞张慧许国云林福呈
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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