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Copper ion detection method and detection kit thereof

A copper ion detection and kit technology, applied in the field of analytical chemistry, can solve the problems of expensive instruments and achieve low cost, non-toxic and good stability

Active Publication Date: 2016-02-24
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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Problems solved by technology

However, the instruments used in these methods are expensive and require professional operators, which limits their use in primary laboratories

Method used

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  • Copper ion detection method and detection kit thereof
  • Copper ion detection method and detection kit thereof
  • Copper ion detection method and detection kit thereof

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[0021] A copper ion detection kit, comprising reducing agent, dNTP, polymerase, detection nucleic acid sequence and buffer system, the detection nucleic acid sequence is composed of nucleic acid sequences A, B, C, D, wherein:

[0022] The nucleic acid sequence A is DNA with a stem-loop structure, and the two ends are respectively modified with a fluorescent group and a quencher group;

[0023] The nucleic acid sequences B and C are complementary to the sequences on the left and right sides of the ring structure of the nucleic acid sequence A respectively, and after pairing, the two adjacent ends are respectively modified with an azide group and an alkynyl group;

[0024] Nucleic acid sequence D is the primer sequence of nucleic acid sequence A.

[0025] As a further improvement of the above copper ion detection kit, the stem length of the nucleic acid sequence A is 6-9 bp, preferably 7 bp. This is because if the sequence is too short, it is difficult to form a loop, and if it...

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Abstract

The invention discloses a copper ion detection method and a detection kit thereof. An azide group of a molecule and an alkynyl group of the other molecule are used, under catalysis of monovalent copper ions, a cycloaddition reaction is carried out to form triazole five-membered ring, and the azide group and the alkynyl group are connected, so that a hairpin structure for opening a fluorescence probe by a nucleic acid sequence is initiated, copper ions detection can be realized through amplification of an isothermal signal, and the detection kit has the advantages of good stability, high sensitivity, good selectivity, strong anti-interference capability, low cost and no toxicity, and strong practicality, and is especially suitable for detection and analysis of an environmental sample.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a copper ion detection method and a detection kit, in particular to a detection method and a detection kit for copper ions based on click chemistry and isothermal signal amplification. Background technique [0002] Copper ions (Cu 2+ ) is one of the essential trace elements for organisms, too high or too low Cu 2+ Will have harmful effects in living organisms. Cu 2+ Excess is usually caused by environmental heavy metal ion pollution, enriched in organisms through the food chain, affecting the normal metabolism of liver and kidney, causing neurodegenerative diseases and causing cancer. Therefore, for Cu 2+ Detection is of great significance in terms of ecological environment protection and food safety. The U.S. Environmental Protection Agency stipulates that Cu in drinking water 2+ The concentration should not exceed 20mM. Traditional Cu 2+ The detection methods are mainly...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 陈俊华周顺桂
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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