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A preparation method and application of biocompatible quantum dots

A surface marker, stem cell technology, applied in the field of preparation of biocompatible quantum dots, can solve the problems of strong cytotoxicity and poor water solubility of QDs, and achieve the effects of reducing cytotoxicity, not easy to quench, and simple equipment and process

Inactive Publication Date: 2018-03-02
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the current problems such as poor water solubility and strong cytotoxicity of QDs, and to provide a preparation method for biocompatible QDs. application in technology

Method used

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  • A preparation method and application of biocompatible quantum dots
  • A preparation method and application of biocompatible quantum dots
  • A preparation method and application of biocompatible quantum dots

Examples

Experimental program
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Embodiment 1

[0046] Embodiment 1: Preparation of CdSe / ZnS-Polymer

[0047] 1. Synthesis of CdSe: Under the protection of nitrogen, 39.5mg of selenium (Se) powder was dissolved in 50mL of liquid paraffin, and the temperature was gradually increased to 200°C. After vigorous stirring for 1 hour, the Se mixture was cooled to about 100°C. Dissolve 0.64g of CdO and 5.7g of stearic acid in 5ml of liquid paraffin, and stir at 160°C under nitrogen protection. When the mixture turns bright yellow, quickly inject the previously synthesized Se mixture and rapidly increase the temperature of the mixture to 200°C. After 90 minutes, the temperature of the mixture was lowered to 60°C, and 50 mL of chloroform was added until thoroughly mixed. The mixture was centrifuged at 8000 rpm for 10 minutes, and the supernatant was discarded. Add 50 mL of ethanol and mix well. After centrifugation again, the pellet was washed again with ethanol. The precipitate was dried and stored in a dark environment to obtai...

Embodiment 2

[0055] Embodiment 2: Preparation of CdSe / ZnS-Polymer

[0056] The difference between Example 2 and Example 1 is that in Step 1, under the protection of nitrogen, 79mg of selenium (Se) powder was dissolved in 50mL of liquid paraffin, and the temperature was gradually increased to 200°C. All the other steps are the same as in Example 1.

Embodiment 3

[0057] Embodiment 3: Preparation of CdSe / ZnS-Polymer

[0058] The difference between Example 2 and Example 1 is that in step 1, under the protection of nitrogen, 158mg of selenium (Se) powder was dissolved in 50mL of liquid paraffin, and the temperature was gradually increased to 200°C. All the other steps are the same as in Example 1.

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Abstract

The present invention relates to a preparation method and application of biocompatible quantum dots, which belong to the technical field of cell labeling. The technical scheme of the present invention is: firstly, the present invention adopts an aqueous phase method to synthesize cadmium selenide (CdSe), and through Zinc sulfide (ZnS) was grown to prepare CdSe / ZnS core-shell QDs with low cytotoxicity. The amphoteric polymer is synthesized, and then the amphoteric polymer is coupled with CdSe / ZnS to obtain a CdSe / ZnS‑Polymer with strong water solubility and good biocompatibility. Due to the strong water solubility and good biocompatibility of QDs coupled with amphoteric polymers, it is possible to use them to fluorescently label living cells and track them in vivo. Using the biocompatible QDs for stem cell labeling and fluorescence imaging of the distribution of QDs in vivo enhanced the fluorescence signal and improved the observation sensitivity.

Description

technical field [0001] The invention belongs to the technical field of cell labeling, and in particular relates to a preparation method and application of biocompatible quantum dots. Background technique [0002] In the research of cell biology, in order to observe structures and molecules of interest, the most common method is to label them with fluorescent light. However, traditional fluorescent probes suffer from many limitations in the field of biomarkers. Due to their special photophysical properties, quantum dots are increasingly widely used as fluorescent probes and play an increasingly important role in the field of biomarkers. [0003] Quantum dots are semiconductor crystals with nanometer dimensions. Compared with traditional fluorescent dyes, quantum dots possess many unique optical properties, such as broad absorption spectrum, narrow and symmetrical emission spectrum, resistance to bleaching, high brightness and long fluorescence lifetime, etc. Due to their e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88C09K11/02C12Q1/02
Inventor 石勇铨刘浩琪崔大祥汤玮
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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