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Tissue culture method for cymbidium tortisepalum and application thereof

A technology of tissue culture and lotus petal orchid, which is applied in the fields of application, horticultural methods, botany equipment and methods, etc., can solve the problem that there is no mature application technology in the application of lotus petal orchid tissue culture, which hinders the industrialized seedling cultivation of lotus petal orchid and Industrial development, poor root quality and other problems, to achieve the effect of saving electricity resources, promoting efficient and rapid tissue culture, and improving efficiency

Active Publication Date: 2016-02-24
广东翁山兰花研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inherent long growth cycle of orchids, low differentiation efficiency, poor root quality and other problems have always hindered the industrialized seedling cultivation and industrial development of lotus petals.
[0004] At present, there are many studies on the application of LEDs in plant tissue culture, but they all focus on species with relatively mature rapid propagation systems, but there is no mature application technology for the application of lotus petal orchid tissue culture.

Method used

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  • Tissue culture method for cymbidium tortisepalum and application thereof
  • Tissue culture method for cymbidium tortisepalum and application thereof
  • Tissue culture method for cymbidium tortisepalum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Optimal proportioning of lotus petal orchid tissue culture medium components

[0028] 1. Proliferation medium

[0029] The aseptic seedlings grown for 14 months after germination of CymbidiumLianpan "Daxuesu" seeds on 1 / 2MS medium (macroelements and trace elements in MS basal medium) were used as explants. The clustered buds were cut into individual plants, and each plant was cut into about 2 cm sections and inoculated into the proliferation medium for proliferation and culture. The medium is 1 / 2MS medium + agar 7g / L as the control group, 6-benzylpurine (6-BA) 1.5-3.5mg / L, naphthaleneacetic acid (NAA) 0.1-0.3mg / L, activated carbon 0.5- 1g / L, sucrose 10-30g / L+agar 7g / L is the optimized group, and the specific ratio is shown in Table 1. pH value = 5.6-5.8.

[0030] Table 1

[0031] Medium formula

6-BA(mg / L)1

NAA(mg / L)

Activated carbon (g / L)

Sucrose (g / L)

CK

0

0

0

15

1

1.5

0.1

0.5

10

...

Embodiment 2

[0052] Example 2 The use effect of optimal culture medium with LED

[0053] 1. Rhizome induction and budding differentiation culture

[0054] 1. Proliferation medium

[0055] Solid medium of 1 / 2MS+6-BA2.5mg / L+NAA0.3mg / L+activated carbon 0.5g / L+sucrose 15g / L (pH=5.6~5.8)+agar 7g / L.

[0056] 2. Inoculation method

[0057] Cut the clustered shoots of lotus petal orchid seedlings (aseptic seedlings grown for 14 months after sowing and germination of lotus petal orchid "Daxuesu") into individual plants, and each plant is cut into sections of about 2 cm and inoculated into the proliferation medium for proliferation and culture. Five buds were inoculated per bottle, and each treatment had 10 bottles.

[0058] 3. Culture conditions

[0059] The photoperiod is 16h / d, and the light intensity is 20μmo1·m -2 ·s -1 , the ambient temperature is 24 ± 1 ℃. In the experiment, five light sources with different light quality ratios (as shown in Table 7) were set up, and the ratios were: ①...

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Abstract

The invention discloses a tissue culture method for cymbidium tortisepalum and application thereof. The method comprises the steps of taking cymbidium tortisepalum aseptic seedling stem tip as an explant, taking a proliferation medium to induce rhizomes to proliferate, taking an LED (light-emitting diode) as a tissue culture light source, and culturing by using blue light; then transferring the obtained rhizomes to a subculture growth medium, inducing multiple shoots to grow and a seedling to quickly develop, taking the LED as the tissue culture light source, and culturing by using red light; inoculating the newly generated seedling to a rooting medium for strong seedling rooting culture, taking the LED as the tissue culture light source, and culturing by using mixed red light and blue light which are matched according to a ratio of (2 to 4):(6 to 8). By adopting the tissue culture method, the problems of low sprouting ratio, slow growth, difficult rooting and the like existing in the prior tissue culture technology are effectively solved; by using LED lamps as the tissue culture light source, 30 to 50 percent of electricity resource is saved, the cost is saved, and the efficiency of industrialized production is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue rapid propagation, in particular to a tissue culture method and application of lotus petal orchid. Background technique [0002] Lotus petal orchid is a large group in China's genus Orchid, mainly produced in Yunnan. Because of its beautiful plant shape, changeable flower shape, rich flower color, and high market price, the wild resources of lotus petal orchid have been destructively excavated, resulting in endangered resources. Therefore, it is particularly important to study the tissue culture and rapid propagation technology of the lotus petal orchid. However, the inherent long growth cycle of orchids, low differentiation efficiency, and poor root quality have always hindered the industrialized seedling cultivation and industrial development of orchids. [0003] Light quality is an important factor affecting plant growth and development. In recent years, studies have shown that differen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨凤玺朱根发王真刘海林黄丹许庆全
Owner 广东翁山兰花研究有限公司
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