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Culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation and method

A culture medium and basal medium technology, applied in the directions of culture medium, planting substrate, horticultural methods, etc., can solve the problems of fast propagation of fish and wood in vitro, limited propagation speed of root burial, low seed germination rate, etc. Improve the availability, avoid blindness, and reduce the effect of the ratio

Active Publication Date: 2017-06-30
广州草木蕃环境科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Yumu usually adopts seed propagation and root burial propagation, but under natural conditions, the germination rate of seeds is very low, and the limited root burial propagation speed limits its large-scale promotion and planting (Tyagi, 2010)
At present, there is no report on the rapid propagation of fish wood in vitro culture

Method used

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  • Culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation and method
  • Culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation and method
  • Culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Effect of BA, NAA and TDZ content in medium 1 used for primary culture on the primary culture of Yumu

[0078] Experimental settings Test groups 1-6, in test groups 1-6, the medium 2 for subculture is: MS medium is used as the basal medium, and 6-benzyl adenine 0.5 mg / L is added , naphthalene acetic acid 0~0.1mg / L, gibberellin 0~0.5mg / L, sucrose 25~35g / L and agar 6.5~7.5g / L; the medium 3 for the strong seedlings is: Using MS medium as the basic medium, and adding 25-35g / L sucrose and 6.5-7.5g / L agar; the medium 4 for rooting is: 1 / 2-1MS medium The basal medium is also supplemented with indole acetic acid 0.5-1.5mg / L, sucrose 25-35g / L and agar 6.5-7.5g / L; the matrix is: peat soil with a volume ratio of 2:1 and A matrix formed by mixing sand; in the medium 1 for primary culture, the content of sucrose is 25-35 g / L, and the content of agar is 6.5-7.5 g / L.

[0079] In the medium kits used in test groups 1 to 6, the medium 2 for subculture, the medium 3 for strong seedli...

Embodiment 2

[0097] BA, NAA and GA in medium 2 for subculture 3 The effect of content on Yumu subculture, and the effect of subculture times on Experiments on the Influence of Bud Propagation of Fish Trees

[0098] Test set-up Test groups 1-4, in test groups 1-4, the medium 1 used for the primary culture is: MS medium is used as the base medium, and 6-benzyl adenine is added with 0.5-2 mg / L , naphthalene acetic acid 0.1mg / L, thiadizuron 0~0.1mg / L, sucrose 25~35g / L and agar 6.5~7.5g / L; the medium 3 for strong seedlings is: use MS The medium is a basal medium, and a medium with 25-35 g / L of sucrose and 6.5-7.5 g / L of agar is added; the medium 4 for rooting is: 1 / 2-1MS medium as a base culture 0.5-1.5 mg / L indole acetic acid, 25-35 g / L sucrose, and 6.5-7.5 g / L agar; The resulting matrix; in the medium 2 for subculture, the content of sucrose is 25-35 g / L, and the content of agar is 6.5-7.5 g / L.

[0099] In the medium kits used in test groups 1-4, the medium 1 for primary culture, the m...

Embodiment 3

[0108] Effect test of the medium 3 used for strong seedlings on the vitrified seedlings of Yumu

[0109] Adopt the method described in Example 1 to carry out the in vitro propagation of Yumu, wherein, the medium 1 for the primary culture is: MS medium is used as the base medium, and 6-benzyl adenine is added with 0.5- 2mg / L, naphthaleneacetic acid 0.1mg / L, thiadiuron 0-0.1mg / L, sucrose 25-35g / L and agar 6.5-7.5g / L; the medium for subculture 2 For: MS medium is used as the basic medium, and 6-benzyl adenine 0.5mg / L, naphthaleneacetic acid 0-0.1mg / L, gibberellin 0-0.5mg / L, sucrose 25-35g / L are added And the culture medium of agar 6.5~7.5g / L; The described medium 3 that is used for strong seedling is: take MS medium as basic medium, also add sucrose 25~35g / L and agar 6.5~7.5g / L culture medium; the medium 4 for rooting is: 1 / 2~1MS medium as the base medium, also added with indole acetic acid 0.5~1.5mg / L, sucrose 25~35g / L and agar 6.5 ~7.5g / L culture medium; the matrix is ​​a m...

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Abstract

The invention discloses a culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation. The culture medium box set comprises a culture medium 1 for primary culture, a culture medium 2 for secondary culture, a culture medium 3 for toughening seedlings and a culture medium 4 for taking roots, wherein the culture medium 1 for primary culture takes an MS (murashige and skoog) culture medium as a basal culture medium, 0.5-2mg / L of BA (butyl acrylate), 0.1mg / L of NAA (naphthalene acetic acid), 0-0.1mg / L of TDZ (thidiazuron), 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium, the culture medium 2 for secondary culture takes an MS culture medium as a basal culture medium, 0.5mg / L of BA, 0-0.1mg / L of NAA, 0-0.5mg / L of GA3 (gibberellin), 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium, the culture medium 3 for toughening seedlings takes an MS culture medium as a basal culture medium, 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium, the culture medium 4 for taking roots takes 1 / 2 MS culture medium as a basal culture medium, and 0.5-1.5 mg / L of IBA (indole-butyric acid), 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium. The invention further discloses a method for facilitating crgotvaunilocularis in-vitro rapid propagation by the culture medium box set. Crgotvaunilocularis is cultured by the method under optimized conditions, and ideal reproduction rate, rooting rate and transplanting survival rate can be achieved.

Description

technical field [0001] The invention relates to a medium box and a method for plant tissue culture, in particular to a medium box and a method for the in vitro rapid propagation of fish tree buds. Background technique [0002] Crateva religiosa G. Forster is a small deciduous tree of the genus Crateva in the family Capparidacea. It has a beautiful tree shape and beautiful flowers. When it is in full bloom, it looks like a group of butterflies flying around. It is suitable for viewing. Its wood can be used for musical instruments and crafts; the fruit contains alkaloids, which can be used as adhesives, and the peel can be used as dyes. [0003] Yumu usually adopts seed propagation and root burial propagation, but under natural conditions, the germination rate of seeds is very low, and the limited root burial propagation speed limits its large-scale promotion and planting (Tyagi, 2010). Rapid propagation in vitro has the advantages of non-season dependence, genetic consistenc...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01G24/00A01H4/001A01H4/008
Inventor 胡秀梁韩枝吴永清邓莎
Owner 广州草木蕃环境科技有限公司
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