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Tissue-culture rapid propagation method for craigia yunnanensis

A technique for tissue culture and rapid propagation of tung tree, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low natural resources of tung tree and the inability to achieve a large number of them, so as to maintain genetic stability and consistency, The effect of high effective reproduction rate and simplified reproduction steps

Active Publication Date: 2020-01-10
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for tissue culture and rapid propagation of Diantong, which solves the problems that Diantong has few natural resources and cannot achieve mass reproduction, preservation and sustainable utilization in a short period of time.

Method used

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  • Tissue-culture rapid propagation method for craigia yunnanensis
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  • Tissue-culture rapid propagation method for craigia yunnanensis

Examples

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Embodiment 1

[0033] Take the young terminal buds and side buds of Diantong as explants, soak them in 1% soapy water for 10 minutes, rinse them with running water, put them on a super-clean table, cut them into stems with single nodes, and use 75% Disinfect with alcohol for 10 seconds, rinse with sterile water for 3 times, disinfect the surface with 0.1% mercuric chloride solution for 5 minutes, wash with sterile water for 3 to 5 times, and inoculate in the prepared co-medium for induction and differentiation of Diantong, 1 per bottle Festival.

[0034] 1. The effect of different induction differentiation media on the induction rate

[0035]The stem segments were inoculated in the following medium: ①MS+0.1mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ②MS+0.5mg / L 6-BA+0.5 mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ③MS+0.8mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ④MS+1mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ⑤MS+2mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, ...

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Abstract

The invention relates to the technical field of plant tissue culture and provides a tissue-culture rapid propagation method for craigia yunnanensis. According to the tissue-culture rapid propagation method, induction and differentiation culture are integrated, multiplication and subculture are integrated, and seedling strengthening and rooting culture are integrated, so that the culture method issimplified. The tissue-culture rapid propagation method has advantages that the problem of difficulty in short-time mass propagation, storage and sustainable utilization due to less natural resourcesof craigia yunnanensis can be solved, a biotechnological research gap of craigia yunnanensis is filled, and a foundation is laid for comprehensive development and sustainable utilization of craigia yunnanensis.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Diantong. Background technique [0002] Craigia yunnanensis belongs to the genus Diantong of Tiliaceae. It is a deciduous tree with a height of 6-20 meters. It is mainly distributed in the subtropical dry-wet alternating hot climate zone and mountain forests at an altitude of 500-1000 meters. The capsule has thin papery wings. Endemic to Southwest China. Diantong is one of the important tree species of the oligospecies genus Diantong, which is of great value in the study of floristic geography and the application of breeding precious tree species. In 2017, it was listed as an endangered EN C2a; D species in the "List of Threatened Species of Higher Plants in China"; it was also listed as an endangered species by the International Union for Conservation of Nature (IUCN) [EN]. At present, there are two ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗桂芬孙卫邦杨静杨佳俊
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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