Aseptic germinating and rapid propagating method for hibiscus aridicola anthony seeds
A seed and hibiscus technology, applied in seed immunization, horticultural methods, seed and rhizome treatment, etc., can solve the problems of difficult introduction and domestication, narrow distribution area of hibiscus in dry land, etc., to restore genetic diversity, simplify reproduction steps, and grow seedlings easy effect
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[0037] 1. Media and matrix screening:
[0038] Take the plump and slightly yellowed fruit of Hibiscus aridum as explants, soak it in 1% soapy water for 10 minutes, rinse it with running water, put it on an ultra-clean table, disinfect it with 75% alcohol for 15 minutes, and rinse it with sterile water for 3 times , remove the peel, take out the seeds, sterilize the surface with 0.1% mercuric chloride solution for 5 minutes, wash with sterile water for 3 to 5 times, inoculate in the prepared Hibiscus upland seed germination medium, 5 capsules per bottle.
[0039]The seeds were inoculated on MS, 1 / 2MS, 1 / 3MS, and improved MS (close to 1 / 4MS) medium, each medium did not contain organic matter and hormones, sucrose 30g / L, agar 5g / L, pH5.8 . After 7 days in dark culture medium and 13 days in light culture, the germination rate of seeds was counted. The light intensity is 1000LUX, and the temperature is 23-30°C.
[0040] Table 1 Screening of seed sterile germination medium
[00...
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