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Immobilized complex microbial agent and method of directly utilizing immobilized complex microbial agent for fermenting sweet sorghum straw to make feedstuff

A technology of sweet sorghum stalks and compound bacterial agents, applied in the biological field, can solve the problems such as difficult control of fermentation flora, and achieve the effects of maintaining the balance of intestinal flora structure, high bacterial count, good fermentation stability and repeatability

Pending Publication Date: 2016-02-17
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide an immobilized composite bacterial agent to solve the problem that the fermentation flora is not easy to control in the current feed process

Method used

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  • Immobilized complex microbial agent and method of directly utilizing immobilized complex microbial agent for fermenting sweet sorghum straw to make feedstuff

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] a. Preparation of MRS medium: 10g of peptone, 10g of beef extract, 5g of yeast extract (dry powder), 2g of dipotassium hydrogen phosphate, 2g of diammonium hydrogen citrate, 3g of sodium acetate trihydrate, 20g of glucose, 1ml of Tween 80, Magnesium sulfate heptahydrate 0.25g, manganese sulfate tetrahydrate 0.05g, calcium carbonate 20g, agar 16-18g, distilled water 1000mL, sterilize by high pressure steam at 121℃ for 20min, pour into sterilized plate and solidify for later use ;

[0036] b. Screening of lactic acid bacteria strains: Take 5g sample from natural silage sweet sorghum stalk feed, put it in sterilized 25mL normal saline and let it stand for 30min, after gradient dilution, spread it on the MRS medium plate prepared above, at 36℃ After anaerobic culture for 72 hours, the colonies of lactic acid bacteria were selected and inoculated in the slant medium for storage. The formula of the slant medium was: 200g of skimmed milk powder, 16-18g of agar, and 1000mL of...

Embodiment 1

[0041] The compounding process of the liquid bacterial agent is as follows. Lactobacillus acidophilus is used as the starting bacterium, and the lactic acid bacteria suspension is mixed with polyvinyl alcohol gel at a volume ratio of 2:8 after expansion, and then placed in the refrigerator to freeze for total Immobilized, cut into blocks after thawing, inserted into sterilized sweet sorghum stalk juice at a ratio of 10-20% and cultured repeatedly, and the immobilized lactic acid bacteria suspension was obtained after the flora structure tended to be stable. After compounding the Candida bacterium liquid, the liquid bacterial agent is obtained, the volume content of the Lactobacillus acidophilus immobilized lactic acid bacteria suspension is 66%, and the volume content of the Candida utilis bacterium liquid is 34%. The number of Lactobacillus acidophilus is 5.0×10 11 individual / mL, the number of Candida utilis was 4.8×10 8 individual / mL.

[0042] 1) Preparation of immobilized...

Embodiment 2

[0044] Embodiment 2: Repeat Example 1, the following difference is arranged: the liquid bacterial preparation immobilizes the lactic acid bacteria suspension with Lactobacillus acidophilus: Candida utilis bacterium liquid=60%: 40% proportioning composition, after removing the juice, sweeten Sorghum stalks were treated with a dilute acid solution of pH=5.5 for 45 minutes, neutralized and dried, crushed and mixed with bran at a ratio of 7:2, added with bacterial agents, covered with film, stacked, and mixed with market bran at a ratio of 15:2. Sell ​​cellulase powder and mix, measure relevant parameter under the same condition with embodiment 1 when fermenting 20d, the results are shown in attached table 1.

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Abstract

The invention discloses an immobilized complex microbial agent and a method of directly utilizing the immobilized complex microbial agent for fermenting sweet sorghum straw to make feedstuff. The immobilized complex microbial agent is formed by compounding lactobacillus acidophilus GSICC 31221 and candida utilis GSICC 51902. The fresh sweet sorghum straw is inoculated with the immobilized mixed microbial agent, the mixture is coated with a film at room temperature to be stacked and fermented for 20d, and then the mature fermented feedstuff is obtained. According to the method, the high adsorption and slow release features of straw powder are utilized, the straw powder is coupled and adsorbed with a complex microorganism mixed microbial agent to form the immobilized microbial agent, and then the immobilized microbial agent is used for the straw to produce the straw feedstuff through direct fermentation; in this way, fermentation of the sweet sorghum straw can be completed within 20d, the content of crude protein in the straw feedstuff is increased by 62%, fermentation time is shortened by 50% compared with a microorganism microbial agent sold in the market, and the immobilized complex microbial agent has the advantages of achieving cyclic utilization, being environmentally friendly and efficient and the like, and will not damage the environment.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to an immobilized composite microbial agent and a method for making feed with it. Background technique [0002] In recent years, with the rapid development of my country's agricultural economy for many years, the structural contradiction between the waste of straw resources in agricultural areas and the shortage of grass-fed livestock feed has become increasingly prominent, restricting the sustainable development of my country's economic society, and straw feed is the solution to this problem effective way. my country's straw feed technology research began in the 1950s and 1960s. Although a lot of results have been achieved, so far the country is still in the application and promotion stage, and the promotion speed is slow. The reasons are, firstly, the core technology of inoculum for straw feed: the breeding of microbial strains and the compounding process of inoculum, the resear...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K10/12A23K10/30C12R1/23C12R1/72
CPCC12N1/16C12N1/20
Inventor 王治业周剑平杜军国季彬祝英
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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