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A kind of breeding method and its application to improve the sugar yield of Paenibacillus

A bacillus and sugar content technology, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of lack of theoretical guidance, high preparation costs, and difficulty in large-scale production, and achieve improved flocculation Efficiency, the effect of increasing the output of flocculant

Active Publication Date: 2018-08-03
NORTHEASTERN UNIV LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, there are still problems such as lack of theoretical guidance, high preparation cost, and difficulty in large-scale production that urgently need to be solved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The breeding method for improving the sugar yield of Paenibacillus and its application to the sedimentation treatment of fly ash wastewater with a concentration of 4g / L and a particle size of fly ash of 0.050~0.074mm are carried out according to the following process steps:

[0035] (1) Inoculate the starting strain of Paenibacillus into common liquid medium, and activate it for 20 hours at a temperature of 25-30°C, a pH of 7.0, and a shaker speed of 140r / min;

[0036] (2) Centrifuge the activated bacterial solution at a speed of 8000r / min for 15min, discard the supernatant, and dilute the precipitated bacteria with sterile saline to obtain a bacterial suspension with a concentration of 100000cfu / ml;

[0037] (3) Take 15uL of the bacterial suspension in step (2) to make a bacterial film, and use a multifunctional plasma mutagenesis system to mutate it. The mutagenesis conditions are: power supply 300W, working airflow 12L / min, plasma The height between the volume emissi...

Embodiment 2

[0045] The breeding method for improving the sugar yield of Paenibacillus and its application to the sedimentation treatment of fly ash wastewater with a concentration of 10g / L and a fly ash particle size of 0.074~0.150mm are carried out according to the following process steps:

[0046] (1) Inoculate the starting strain of Paenibacillus into common liquid medium, and activate it for 30 hours at a temperature of 25-30°C, a pH of 6.55, and a shaker speed of 130r / min;

[0047] (2) Centrifuge the activated bacterial solution for 10 minutes at a speed of 9000r / min, discard the supernatant, and dilute the precipitated bacteria with sterile saline to obtain a bacterial suspension with a concentration of 100000cfu / ml;

[0048] (3) Take 20uL of the bacterial suspension in step (2) to make a bacterial film, and use a multifunctional plasma mutagenesis system to mutate it. The mutagenesis conditions are: power supply 300W, working airflow 12L / min, plasma The height between the volume emis...

Embodiment 3

[0056] The breeding method for improving the sugar yield of Paenibacillus and its application to the sedimentation treatment of pure culture of Nostoc in water are carried out according to the following process steps:

[0057] (1) Inoculate the starting strain of Paenibacillus into common liquid medium, and activate it for 24 hours at a temperature of 25-30°C, a pH of 7.5, and a shaker speed of 160r / min;

[0058] (2) Centrifuge the activated bacterial solution at a speed of 8000r / min for 15min, discard the supernatant, and dilute the precipitated bacteria with sterile saline to obtain a bacterial suspension with a concentration of 100000cfu / ml;

[0059] (3) Take 15uL of the bacterial suspension in step (2) to make a bacterial film, and use a multifunctional plasma mutagenesis system to mutate it. The mutagenesis conditions are: power supply 300W, working airflow 12L / min, plasma The height between the volume emission source and the sample is 5mm, the working gas is nitrogen, an...

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Abstract

The invention provides a breeding method and application for increasing the sugar production of Paenibacillus. The method is: (1) inoculating the starting strain of Paenibacillus into a common liquid culture medium for activation; (2) diluting it to a certain concentration Bacterial suspension; (3) Make a bacterial film and then carry out mutagenesis; (4) Coat it in an ordinary solid medium and culture it; (5) Preliminary screening; (6) Inoculate the initially screened strain into an ordinary liquid medium and culture it Obtain a strain with higher sugar production; (7) determine that the Paenibacillus strain obtained in step (6) is a Paenibacillus strain with higher sugar production; (8) determine the genetic stability of sugar production of the strain with higher sugar production. The breeding method is applied to the sedimentation treatment of fly ash wastewater and microalgae in water bodies. The method of the present invention uses plasma mutagenesis technology to carry out mutation breeding of Paenibacillus with high yield of microbial flocculant, effectively improves the flocculation efficiency and flocculant production of Paenibacillus, and can be applied to the sedimentation of fly ash wastewater and microalgae in water bodies. deal with.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a breeding method for improving the sugar production of Paenibacillus and its application. Background technique [0002] Microbial flocculant is a safe and easily biodegradable new water treatment flocculant obtained by enrichment, screening and purification from microorganisms or their secretions by using biotechnology. Microbial flocculant is a natural flocculant. It also has spectral flocculation activity under the premise of ensuring application safety, which provides broad application prospects for water supply and drainage treatment, food processing and fermentation industries that are closely related to human health. [0003] With the massive discharge of industrial waste water, water pollution has become increasingly serious, and water environment problems have aroused widespread concern. Flocculation and sedimentation is a water treatment m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/01C02F3/34C12R1/01
Inventor 胡筱敏姜彬慧庞宁黄永刚张兰兰孙伟丽张佳琪
Owner NORTHEASTERN UNIV LIAONING
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