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DNA immunity adsorbent and preparation method thereof

A technology of immunoadsorbent and adsorbent carrier, which is applied in the field of DNA immunosorbent and its preparation, can solve the problems of low adsorption efficiency, complex process, and non-uniform coating thickness of collodion, and achieve stable properties and excellent preparation methods. simple and easy effects

Inactive Publication Date: 2016-02-03
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems of non-uniform thickness and low adsorption efficiency of the envelope of collodion used in the DNA immunosorbent in the prior art, the first purpose of the present invention is to provide a DNA immunosorbent
[0006] Aiming at the problem of complicated process in the preparation method of DNA immunosorbent in the prior art, the second object of the present invention is to provide a kind of preparation method of DNA immunosorbent with simple preparation method

Method used

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  • DNA immunity adsorbent and preparation method thereof
  • DNA immunity adsorbent and preparation method thereof
  • DNA immunity adsorbent and preparation method thereof

Examples

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preparation example Construction

[0038] In the embodiment of the present invention, the preparation method of the DNA immunoadsorbent is as follows. Based on the difference in the degree of crosslinking, the preparation method specifically includes two preferred schemes. Wherein, when the theoretical degree of cross-linking of polyvinyl alcohol is less than 15%, then follow the steps of the first method, the steps of the first method are as follows: Execution of step A: first, add the cross-linking agent in the polyvinyl alcohol aqueous solution Cross-linking reaction with the catalyst to obtain a cross-linked polyvinyl alcohol solution; then, add the DNA solution to the cross-linked polyvinyl alcohol solution; then, add the adsorbent carrier, after stirring, the DNA is loaded on the adsorbent carrier, and on the adsorbent carrier A cross-linked polyvinyl alcohol film is formed, and the cross-linked polyvinyl alcohol film forms a coating on the adsorbent carrier; then perform step C, filter with a filter bag,...

Embodiment 1

[0044] Embodiment 1: the preparation process of macroporous adsorption tree is as follows.

[0045] At room temperature, dissolve gelatin in water to make a reaction water phase with a mass fraction of 0.5% to 5%, heat it to 30°C to 50°C, and use a mixed monomer of styrene and divinylbenzene as a reaction oil phase, of which two Vinylbenzene accounts for 0.5% to 1.5% of the total mass of the mixed monomer, the volume ratio of the water phase to the oil phase is 3:1, and benzoyl peroxide is used as the initiator, and the amount of the initiator is 1% of the total mass of the mixed monomer- 3%. After suspension polymerization, first slowly raise the temperature to 70°C to 80°C and react for more than 3 hours, then slowly raise the temperature to 85°C for 3 hours, then slowly raise the temperature to above 95°C and react for 3 hours, then filter and wash to prepare A low-crosslinked gel-type polystyrene resin, referred to as white ball.

[0046] Add white balls into the there-n...

Embodiment 2

[0053] Example 2: Compared with Example 1, this example replaces maleic anhydride with a high-purity medical-grade glutaraldehyde aqueous solution with a concentration of 50% (the concentrations of the glutaraldehyde aqueous solutions in the following examples are all 50%) , the other steps were the same as in Example 1 to prepare a DNA immunosorbent with a cross-linking degree of 10%.

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Abstract

The invention relates to DNA immunity adsorbent and a preparation method thereof. The DNA immunity adsorbent comprises an adsorbent carrier loaded with DNA. The DNA immunity adsorbent further comprises a cross-linking polyvinyl alcohol film mixed with DNA to achieve a film wrapping effect for the adsorbent. The preparation method includes the following step of sequentially conducting cross-linking and film wrapping or sequentially conducting film wrapping and cross-linking. The DNA immunity adsorbent has adsorption performance for anti-double stranded DNA antibodies in SLE patient plasma, and is superior to DNA immunity adsorbent prepared from a collodion wrapping film in general.

Description

technical field [0001] The invention relates to the field of blood purification, in particular to a DNA immunoadsorbent coated with cross-linked polyvinyl alcohol (PVA) and DNA and a preparation method thereof. The DNA immunoadsorbent has specific adsorption for ds-DNA . Background technique [0002] Systemic lupus erythematosus (systemic lupus erythematosus, SLE) is a relatively common autoimmune disease involving multiple systems and organs. Due to the immune regulation disorder of the patient's body and the breakdown of autoimmune tolerance, a variety of autoantibodies are produced. The pathogenesis is mainly due to immune complex formation. Various studies have confirmed that anti-double-stranded DNA antibodies (anti-ds-DNA antibodies) are positively correlated with SLE disease activity. Especially in patients with lupus nephritis, anti-ds-DNA antibodies combine with DNA to form immune complexes and deposit on the glomerular basement membrane, or ds-DNA antibodies dire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30B01J20/28A61M1/36
CPCA61M1/362A61M1/3621A61M1/3689A61M2210/12B01J20/26
Inventor 董凡黄杰辉杜鸿雁
Owner JAFRON BIOMEDICAL
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