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Preparation method of feed micro-ecologic agent produced by using citrus peels

A micro-ecological preparation, citrus peel technology, applied in the field of feed industry and animal husbandry, can solve the problems of resource waste, animal feed bitterness, environmental pollution, etc., to save pollution control costs, create economic benefits, and improve competitiveness. Effect

Inactive Publication Date: 2016-02-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Citrus peels have nutritional and medicinal value and are rich in carbohydrates, fats, vitamins, amino acids and minerals. However, undried citrus peels have a high water content and are easy to rot and deteriorate, so they are not suitable for long-distance transportation and Long-term storage, discarding is easy to cause waste of resources and pollution to the environment
At present, most of the citrus peels in China are discarded and discarded by landfill, and only a small part is dried as Chinese herbal medicine or silaged into animal feed. The absorption is not good and it is difficult to industrialize, so the utilization, research and innovation of citrus peels are imperative

Method used

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  • Preparation method of feed micro-ecologic agent produced by using citrus peels
  • Preparation method of feed micro-ecologic agent produced by using citrus peels
  • Preparation method of feed micro-ecologic agent produced by using citrus peels

Examples

Experimental program
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Effect test

Embodiment 1

[0019] 1) Inoculate Lactobacillus delbrueckii (preservation number CCTCCM207096), Bacillus subtilis (preservation number CCTCCM207097), mirabilis (preservation number CCTCCM207098) into 100ml MRS liquid medium, 100ml broth liquid medium, 100ml potato dextrose liquid medium respectively , cultured at a constant temperature of 37°C for 24 hours to obtain a seed solution;

[0020] 2) Air-dried 100 parts of citrus peel, 10 parts of brewer's grains, 15 parts of wheat bran, 3 parts of glucose, 8 parts of soybean meal, 2 parts of urea, 2 parts of ammonium sulfate, 0.2 parts of potassium dihydrogen phosphate, 0.3 parts of magnesium sulfate according to weight ratio Parts were crushed separately, passed through a 40-mesh sieve, mixed evenly, added 100 parts of distilled water, mixed thoroughly, sterilized at 121°C for 15 minutes, cooled to room temperature for later use, and obtained a solid medium;

[0021] 3) Mix the three seed liquids prepared above at a volume ratio of 1:1:1, add t...

Embodiment 2

[0025] 1) Inoculate Lactobacillus delbrueckii (preservation number CCTCCM207096), Bacillus subtilis (preservation number CCTCCM207097), mirabilis (preservation number CCTCCM207098) into 100ml MRS liquid medium, 100ml broth liquid medium, 100ml potato dextrose liquid medium respectively , cultured at a constant temperature of 30°C for 21 hours to obtain a seed solution;

[0026] 2) Air-dried 100 parts of citrus peel, 12 parts of brewer's grains, 17 parts of wheat bran, 4 parts of glucose, 9 parts of soybean meal, 2.5 parts of urea, 2.5 parts of ammonium sulfate, 0.35 parts of potassium dihydrogen phosphate, and 0.35 parts of magnesium sulfate according to the weight ratio. Parts were crushed separately, passed through a 40-mesh sieve, mixed evenly, added 110 parts of distilled water, mixed thoroughly, sterilized at 121°C for 30 minutes, cooled to room temperature for later use, and obtained a solid medium;

[0027] 3) Mix the three seed solutions prepared above at a volume rati...

Embodiment 3

[0031] 1) Inoculate Lactobacillus delbrueckii (preservation number CCTCCM207096), Bacillus subtilis (preservation number CCTCCM207097), mirabilis (preservation number CCTCCM207098) into 100ml MRS liquid medium, 100ml broth liquid medium, 100ml potato dextrose liquid medium respectively , cultured at a constant temperature of 34°C for 18 hours to obtain a seed solution;

[0032]2) Air-dried 100 parts of citrus peel, 15 parts of brewer's grains, 20 parts of wheat bran, 5 parts of glucose, 10 parts of soybean meal, 3 parts of urea, 3 parts of ammonium sulfate, 0.5 parts of potassium dihydrogen phosphate, 0.4 parts of magnesium sulfate according to the weight ratio Parts were crushed separately, passed through a 40-mesh sieve, mixed evenly, added 120 parts of distilled water, mixed thoroughly, sterilized at 121°C for 20 minutes, cooled to room temperature for later use, and obtained a solid medium;

[0033] 3) Mix the three seed solutions prepared above at a volume ratio of 2:1:2,...

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Abstract

The present invention discloses a preparation method of a feed micro-ecologic agent produced by using citrus peels and a product thereof. The preparation method: Lactobacillus delbrueckii (Preservation No., CCTCC M 207096), Bacillus subtilis (Preservation No., CCTCC M 207097), Saccharomyces paradoxus (Preservation No., CCTCC M 207098) are inoculated to the corresponding liquid culture medium and cultured at a constant temperature of 37 DEG C for 18-24 hours, then the prepared bacteria solution is mixed in a certain proportion, the mixture is inoculated into sterilized solid state fermentation medium with the citrus peel as the main raw material and fermented at 30-40 DEG C for 36-48 hours, the fermented mixture is dried at a low temperature of 50-55 DEG C, and the dried mixture is crushed, thereby obtaining the feed micro-ecologic agent. Production technology of the feed micro-ecologic agent has the advantages of low investment, low energy consumption, no waste water pollution, etc., and opens up a new way for the utilization of the citrus peels at the same time.

Description

technical field [0001] The invention relates to the fields of feed industry and animal husbandry, in particular to a preparation method of using citrus peels to produce feed microecological preparations. Background technique [0002] Since the discovery in the middle of the 20th century that subtherapeutic doses of antibiotics added to feed have a growth-promoting effect, antibiotics have been widely used in animal husbandry and have brought certain economic benefits to animal husbandry. At present, whether it is the prevention or treatment of livestock and poultry diseases, antibiotics are still basically used. However, the long-term use of antibiotics in large quantities has led to increasingly prominent problems such as drug resistance of pathogenic microorganisms, drug residues in livestock products, and the decline in the immune system of livestock and poultry, resulting in more frequent occurrence of livestock and poultry diseases, and an increase in the mortality rate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12
Inventor 黄兴国彭伟贺建华黄其永
Owner HUNAN AGRICULTURAL UNIV
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