Method for rescuing grown-up seedlings of special precocious peach hybrid embryos

Abstract

The invention discloses a method for rescuing grown-up seedlings of special precocious peach hybrid embryos, wherein seven to eight cooked fruits of special precocious peaches are picked, ovules are taken out by breaking nucleus after the seven to eight cooked fruits are disinfected, and are inoculated in a growth medium to culture, then young embryos are inoculated into a differential medium to differentiate and culture, and embryos which are broken in dormancy are germinated and cultured, and are transplanted. The method for rescuing the grown-up seedlings of the special precocious peach hybrid embryos is suitable in rescue time of the special precocious peach hybrid embryos, develops a suitable culture medium during the ovule culture and embryos germination process, improves developmental rate of hybrid embryos, improves normal radio of embryos, is tidy and healthy in embryo rescue seedlings, can achieve above 83% embryo growth force, achieves 60% dissolution power, achieves 50% culture ability, and greatly improves cardinal number of hybrids embryo rescue seedlings of special precocious peaches, thereby being capable of obtaining more hybrids plant groups of special precocious peaches, improving hybrids embryo rescue seedling rate of the special precocious peaches, and being strong in maneuverability and easy to master.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for rescuing hybrid embryos of super early maturing peaches into seedlings. Background technique [0002] Fruit maturity is an important economic trait in breeding. Southern my country enters the rainy season in June. Most of the ripe peaches during this period are of poor quality due to the weather, and the rainy season affects the public’s ability to purchase peaches, which restricts the development of early-ripening peaches in Shanghai. In the first ten days of June, the weather is fine and there is little rain. If the ripening period of early-maturing peaches can be advanced to this time, it will enrich the resources of peach varieties in my country, increase the supply of peach fruit market, and promote the development of the peach industry in the southern region. [0003] Peach matures early, has good quality, labor-saving cultivation and management, and hig...

AI Technical Summary

Problems solved by technology

[0006] However, in the process of embryo rescue, the genotype of the variety, the time of sampling and inoculation, the type and phase of the medium, plant growth regulators, carbon sources, culture environment, transplanting methods, etc. will all have an impact on the germination rate and seedling rate of embryo rescue. Influenced by various factors, and the interaction of various factors, because the seed embryos of extra-early peaches are very small, and abortion occurs during the development process, the embryo culture process needs to let the embryos grow up first and then germinate into seedlings. The operation is complicated and the seedling rate is low. Severely limit the breeding efficiency of extra early maturing peach

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