Method for measuring number of circulating epithelial tumor cells in blood
A tumor cell and epithelial technology, applied in the field of concentration measurement, can solve the problem of loss or destruction of circulating tumor cells
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[0030] In order to have a further understanding and understanding of the structural features of the present invention and the achieved effects, the detailed description of the preferred embodiment is as follows:
[0031] A method for measuring the number of circulating epithelial tumor cells in blood, said method comprising the steps of:
[0032] A. The blood sample of the test item is obtained from the test subject. The sample is anticoagulated whole blood, which is suspected to contain epithelial tumor cells; B. Negative enrichment removes non-target cells in the blood sample, which include red blood cells and most white blood cells;
[0033] C. Mix the enriched blood sample with the fluorescently labeled antibody and incubate to fully combine the target cells with the fluorescent antibody;
[0034] D. Apply the fluorescently labeled cell suspension to the anti-slip slide, let it stand for a while, observe and analyze the sample under a fluorescent microscope, so as to deter...
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