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Method for measuring number of circulating epithelial tumor cells in blood

A tumor cell and epithelial technology, applied in the field of concentration measurement, can solve the problem of loss or destruction of circulating tumor cells

Inactive Publication Date: 2016-01-27
SHANG OUTDO BIOTECH CO LTD
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Problems solved by technology

[0005] The purpose of the present invention is to solve the defect that the enrichment process of circulating tumor cells in the prior art is easy to cause the loss or destruction of circulating tumor cells due to various reasons, and to provide a method for measuring the number of circulating epithelial tumor cells in blood to solve the above-mentioned problems. question

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Embodiment Construction

[0030] In order to have a further understanding and understanding of the structural features of the present invention and the achieved effects, the detailed description of the preferred embodiment is as follows:

[0031] A method for measuring the number of circulating epithelial tumor cells in blood, said method comprising the steps of:

[0032] A. The blood sample of the test item is obtained from the test subject. The sample is anticoagulated whole blood, which is suspected to contain epithelial tumor cells; B. Negative enrichment removes non-target cells in the blood sample, which include red blood cells and most white blood cells;

[0033] C. Mix the enriched blood sample with the fluorescently labeled antibody and incubate to fully combine the target cells with the fluorescent antibody;

[0034] D. Apply the fluorescently labeled cell suspension to the anti-slip slide, let it stand for a while, observe and analyze the sample under a fluorescent microscope, so as to deter...

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Abstract

The invention relates to a method for measuring the number of circulating epithelial tumor cells in blood. The method comprises the following steps that A, a blood sample of a test item is obtained from a test object, wherein the sample is anticoagulant whole blood possibly containing the epithelial tumor cells; B, negative enrichment is performed to remove non-target cells in the blood sample, and the non-target cells include red blood cells and most white blood cells; C, the enriched blood sample is mixed with a fluorescently-labeled antibody, and incubation is performed, so that target cells and a fluorescent antibody are sufficiently combined; D, an anti-fall glass sheet is coated with fluorescently-labeled cell suspension, still standing is performed for a moment, and observation is performed under a fluorescence microscope for analyzing samples, so that the existence and the number of the circulating epithelial tumor cells are determined; E, the observed target cells are stored as direct visual images. Compared with the prior art, the method has the beneficial effect that detection is favorable for tumor early-stage diagnosis, transfer and recurrence monitoring, curative effect and prognosis evaluation, drug-resistance monitoring, individual treatment guide and the like.

Description

technical field [0001] The present invention relates to a method for quantifying the concentration of important epithelial tumor cells in the blood of patients with epithelial tumors, in particular to a method for measuring the number of circulating epithelial tumor cells in the blood. Background technique [0002] Humans have recorded the existence of tumors for more than 3000 years. According to statistics, more than 100 kinds of tumors have been discovered by people. Any part of the human body may be invaded by tumors, and malignant tumors have become the first or second cause of human death. Epithelial malignancies are the most common form of cancerous solid tumors. The cause of death of most cancer patients is not the primary tumor, but the metastasis of the tumor. However, early metastasis and recurrence are often overlooked in the initial diagnosis. [0003] The present invention relates to indicating the presence of a solid tumor. Metastasis and recurrence of so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/533
CPCG01N33/56966G01N33/533
Inventor 王超郜恒骏张宝峰周雁霞张小燕
Owner SHANG OUTDO BIOTECH CO LTD
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