Device and method for gathering anaerobic ammonium oxidation bacteria

A technology for the enrichment of anammox bacteria, applied in the field of enrichment devices for anammox bacteria, can solve the problems of long doubling time, low affinity constant, inhibition of anammox bacteria activity, etc., and achieve enhanced processing capacity , Good interception effect, good treatment effect

Active Publication Date: 2016-01-27
SHENZHEN WATER GRP CO LTD
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Problems solved by technology

[0004] However, the growth of anammox bacteria is slow, the doubling time is long, and they are sensitive to oxygen, but if the oxygen partial pressure is too high, the activity of anammox bacteria may be inhibited
Moreover, the affinity constant of anammox bacteria to ammonium salt and nitrite substrate is low, and the growth of the substrate will be inhibited if the concentration of the substrate is too high.
Therefore, the start-up of the anammox reactor is slow, it is difficult to achieve pure culture, the loss rate of anammox bacteria is high, and its process application is limited

Method used

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  • Device and method for gathering anaerobic ammonium oxidation bacteria
  • Device and method for gathering anaerobic ammonium oxidation bacteria

Examples

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Effect test

Embodiment 1

[0039] Such as figure 1As shown, an enrichment device for anammox bacteria comprises a device body 1, the device body 1 is cylindrical, the top of the device body 1 is open, the bottom of the device body 1 is funnel-shaped, and the upper part of the device body 1 is arranged There is a soft packing layer 2, which is used to prevent the loss of granular sludge. A heating rod 3 is inserted into the top of the device body 1, and the heating rod 3 passes through the soft packing layer 2 to control the water temperature in the device body 1, heating The heating temperature of rod 3 was 35°C. The bottom of the device body 1 is provided with a water inlet pipe 4, and the water inlet pipe 4 is connected to the water tank 10 through the water inlet pump 9. At the soft packing layer 2 of the device body 1, a return pipeline 5 is provided, and the return pipeline 5 passes through the return pump 6. Enter the water inlet pipe 4 through the tee 7. The upper part of the device body 1 is p...

Embodiment 2

[0049] Using the device of Example 1, the bottom sludge of a landfill plant is used as the inoculated sludge, and the simulated wastewater is used as the substrate, and the NH in the influent 4 + -N and NO 2 - -N ratio is 1:1.2, volume load is 0.05kgNH respectively 4 + -N / (m 3 d) and 0.06kgNO 2 - -N / (m 3 d), the pH range is 7.5-8.2, and the heating temperature is 35°C. When the reactor was running for 70 days and 170 days, the average concentrations of ammonia nitrogen, nitrite nitrogen and total nitrogen in the influent and effluent water were detected, as shown in Table 1 for details.

[0050] The enrichment device operation table of the anammox bacteria of table 1 embodiment 2

[0051]

[0052] It can be seen from Table 1 that after 170 days, in the granular sludge at the bottom of the reactor, the anammox bacteria occupy an absolute advantage, indicating that the device has successfully enriched the anammox bacteria.

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Abstract

The invention provides a device and a method for gathering anaerobic ammonium oxidation bacteria. The device for gathering anaerobic ammonium oxidation bacteria comprises a device body, wherein a packing layer is arranged on the upper part of the device body; an anaerobic ammonium oxidation bacteria gathering area is arranged under the packing layer; a heating device is arranged in the anaerobic ammonium oxidation bacteria gathering area; a water inlet pipe is arranged on the lower part of the device body; the water inlet pipe is connected with a water tank; a backflow water outlet pipe is arranged on the packing layer or under the packing layer on the device body; the backflow water outlet pipe is connected with the water inlet pipe; a water draining pipe is arranged above the backflow water outlet pipe on the device body. According to the technical scheme provided by the invention, the structure is simple, the gathering for the anaerobic ammonium oxidation bacteria is high-efficiently realized, the loss ratio of the cultured anaerobic ammonium oxidation bacteria is low, water circulation is utilized to automatically realize the homogenization of temperature, and the energy-saving and emission-reducing effects are achieved.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to an enrichment device and method for anammox bacteria. Background technique [0002] Anammox bacteria is a newly discovered anaerobic autotrophic bacteria, which can convert ammonia nitrogen and nitrite nitrogen into nitrogen at a ratio of 1:1.32. Anammox bacteria have special cell structure and physiological characteristics. Its scientific research value and development value are excellent, and it has attracted much attention from the biological and environmental engineering circles. [0003] The traditional biological denitrification process mainly includes two stages of nitrification and denitrification. These two processes operate independently and need to be carried out in different reaction structures. In the nitrification stage, nitrifying bacteria need a large amount of oxygen to convert ammonia nitrogen into nitrite nitrogen. For nitrate nitroge...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/36
CPCC12M23/20C12M23/22C12M29/02C12M41/24C12N1/36
Inventor 张金松王越兴
Owner SHENZHEN WATER GRP CO LTD
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