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Method for rapid separation of Ligusticum chuanqiong polysaccharide

A Ligusticum chuanxiong polysaccharide and fast technology is applied in the field of separation and extraction of biological macromolecules, which can solve the problems of unpublished patents, complicated procedures, and consumption of reagents for the separation and extraction of chuanxiong polysaccharides, so as to shorten the extraction time, improve the extraction rate, and improve the purity. Effect

Inactive Publication Date: 2016-01-27
XIHUA UNIV
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Problems solved by technology

Therefore, the research on the function and application of Chuanxiong polysaccharide has broad prospects. At present, there are alkaline method, ultrasonic-assisted method, microwave-assisted method and enzymatic method for the separation and extraction of Chuanxiong polysaccharide. time consuming reagents
There is no report on the extraction of Chuanxiong polysaccharides by two-phase system, and no patents on the separation and extraction of Chuanxiong polysaccharides have been published.

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Embodiment Construction

[0022] In order for those skilled in the art to better understand the technical solutions provided by the present invention, the following will be described in conjunction with specific embodiments.

[0023] A kind of method for fast separation of Rhizoma Chuanxiong polysaccharide of the present invention, comprises the steps:

[0024] (1) Dry the root tubers of Rhizoma Chuanxiong at 60°C to constant weight, pulverize them with a high-speed tissue grinder to obtain Rhizoma Chuanxiong powder, and pass the powder through a 40-mesh sieve to obtain Rhizoma Rhizoma Chuanxiong powder;

[0025] (2) Add the powder into the two-phase system according to the solid-to-liquid ratio of 1:40 to make an extraction solution. The two-phase system is composed of 32% ethanol and 24% ammonium sulfate, and the pH of the system is 6.0;

[0026] (3) Put the extraction solution in a microwave extraction device, extract at 400w, 40°C for 15min;

[0027] (4) Centrifuge the extracted liquid after irrad...

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Abstract

The invention relates to a method for rapid separation of Ligusticum chuanqiong polysaccharide, and refers in particular to extraction of Ligusticum chuanqiong polysaccharide through a microwave-assisted double-water-phase system. Ligusticum chuanqiong root tubers are subjected to drying, crushing and degreasing, mixing with an ethanol-inorganic salt double-water-phase extracting solution is carried out, microwave extraction, centrifugation and separation of the alcohol phase are carried out, the alcohol phase is subjected to ethanol precipitation and drying, and Ligusticum chuanqiong polysaccharide is obtained. In the method, extraction of Ligusticum chuanqiong polysaccharide is carried out through microwave-assisted double-water-phase, polysaccharide is dissolved in an organic solvent fully in a short time, extraction time is saved greatly, proteins and color can be removed while Ligusticum chuanqiong polysaccharide is extracted, the polysaccharide recovery rate is more than 80%, the protein removal rate is more than 90%, and the prepared Ligusticum chuanqiong polysaccharide has high activity at the aspect of removing hydroxyl radicals.

Description

technical field [0001] The invention relates to the field of separation and extraction of biological macromolecules, in particular to a method for rapid separation of polysaccharides from Rhizoma Chuanxiong. Background technique [0002] Ligusticum chuanxiong Ligusticumchuanxiong The dried rhizome of Hort has the effects of invigorating blood and promoting Qi, dispelling wind and relieving pain. Ligusticum chuanxiong is a traditional Chinese medicine in our country, and it is also a well-known authentic medicinal material produced in Sichuan. According to statistics, the annual output of chuanxiong is 5×10 6 ~6×10 6 kg, among the 564 prescription preparations and single preparations recorded in Part I of the 2005 edition of the "Chinese Pharmacopoeia", 85 use Chuanxiong, accounting for about 15% of the Chinese patent medicines recorded in the Pharmacopoeia. Ligusticum chuanxiong contains a variety of compounds such as lactones, alkaloids, phenols, and volatile oils. In re...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王维香蒋楠
Owner XIHUA UNIV
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