Protein capable of improving salt tolerance and drought tolerance of plants as well as coding gene and application of protein
A drought tolerance and plant technology, applied in the direction of plant genetic improvement, application, plant peptides, etc., can solve the problem that the mechanism of action remains to be elucidated
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[0045] Embodiment 1, cultivate transgenic rice
[0046] 1. Construction of recombinant expression vector
[0047] 1. Cloning of MePMP3-2 gene
[0048] ① Extraction of cassava total RNA
[0049] After the plant tissue material is collected, cut it into pieces, put it in liquid nitrogen and grind it into a dry powder quickly, take about 100 mg of the powder material, put it into a 1.5 mL centrifuge tube filled with 1.0 mL TRIzol extract (Invitrogen) in advance, and cover it tightly Cap the tube and shake to mix the sample and TRIzol extract thoroughly. After numbering with a low-temperature marker pen, store in a -70°C ultra-low temperature freezer for future use.
[0050] Take out the samples frozen in the ultra-low temperature refrigerator at -70°C for 5 minutes to completely lyse the nucleoprotein of the samples, add an appropriate amount of chloroform according to 200 μl chloroform / ml TRIzol, and shake vigorously for 15 seconds (note: Vortex shaker is not allowed here, I...
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