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Chinese chive seed antioxidative hexapeptide as well as preparation method and application thereof

A leek seed, anti-oxidation technology, applied in the food field, can solve the problem of no reports of active peptides

Inactive Publication Date: 2016-01-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There has been no research on the chemical composition and efficacy of leek seeds abroad, and there are not many domestic researches on chemical composition. So far, there are almost no reports on its active peptides or pharmacological research at home and abroad.

Method used

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  • Chinese chive seed antioxidative hexapeptide as well as preparation method and application thereof
  • Chinese chive seed antioxidative hexapeptide as well as preparation method and application thereof
  • Chinese chive seed antioxidative hexapeptide as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The separation and purification of the antioxidant hexapeptide in the present invention includes two steps of SephadexG-25 gel filtration chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC).

[0019] Preparation of anti-oxidant hexapeptide hydrolyzate of leek seed: using acid protease under its optimal hydrolysis conditions (pH3.02, enzyme dosage 1345.7U, substrate concentration 2% (w / v)) for 6.45h , inactivated in a boiling water bath for 10 minutes, centrifuged at 12000 rpm for 20 minutes, and the supernatant was taken to freeze-dry, which was the leek seed protein enzymatic hydrolysis product;

[0020] SephadexG-25 Gel Filtration Chromatography: Dissolve the lyophilized powder of leek seed enzymatic hydrolyzate in deionized water, and centrifuge at 12000rpm for 15min. The supernatant was filtered through a 0.22 μm pore size microfiltration membrane. SephadexG-50 gel column (1.6cm×100cm) was equilibrated with deionized water, and the fi...

Embodiment 2

[0025] The natural antioxidant hexapeptide activity obtained in Example 1 is studied:

[0026] DPPH free radical scavenging effect: 0.4mL samples with different concentrations and an equal volume of 4% ( W / V ) DPPH anhydrous ethanol solution was mixed thoroughly, kept at room temperature in the dark for 30 minutes, and the absorbance value was measured at 517nm. Deionized water and reduced glutathione were used instead of samples as blank control and positive control, respectively. The DPPH free radical scavenging activity of the sample is calculated according to the following formula (1):

[0027]

[0028] In the formula, A 0 : Absorbance value of blank control group; A s : Absorbance value of sample group

[0029] ABTS free radical scavenging effect: prepare 7mmol / L ABTS with distilled water + solution and 2.45mmol / L potassium persulfate solution (must be placed at room temperature for 16h before use), ABTS + Mix with potassium persulfate solution at a volume ratio...

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Abstract

The invention discloses Chinese chive seed antioxidative hexapeptide as well as a preparation method and application thereof. The sequence of the antioxidative hexapeptide is Phe-Pro-Leu-Pro-Ser-Phe (FPLPSF). In-vitro experiments show that the polypeptide has the effects of effectively removing free radicals such as DPPH, ABTS, hydroxyl radicals, superoxide anions and the like. The antioxidative hexapeptide has the characteristics of simple structure, high antioxidative activity and the like, can be used as a good substitute of a conventional artificially-synthesized antioxidant and is significant for the development and application of novel antioxidative health products.

Description

technical field [0001] The invention relates to a natural anti-oxidation hexapeptide of chive seeds, a preparation method thereof and an application in the food field, belonging to the food field. Background technique [0002] my country is a large agricultural country with a wide variety of crops. However, due to the limitation of processing technology, the processing of crops is mostly in the primary stage, and there is still a relatively large amount of protein in the residues after processing, and most of them are mainly used for feed. Industrial or natural emissions not only waste resources, but also cause environmental pollution. The introduction of the concept of plant active peptides and the improvement of separation and detection technology have made people gradually pay attention to the deep processing of agricultural by-products rich in protein and obtain a variety of active peptides, such as casein phosphopeptides, blood pressure-lowering peptides and easily diges...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/16C07K1/20A23L33/00
Inventor 洪晶陈涛涛张杨杨娟娟叶南慧孟春
Owner FUZHOU UNIV
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