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Deer fetus DNA identification kit and method

An identification method and a technology of a detection kit, which are applied in the field of deer fetal DNA identification kits and identification, can solve the problems of cumbersome operation and inability to accurately identify the authenticity, and achieve reliable detection results

Inactive Publication Date: 2016-01-13
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only has relative identification significance, cannot accurately identify the authenticity, and the operation is cumbersome

Method used

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  • Deer fetus DNA identification kit and method
  • Deer fetus DNA identification kit and method
  • Deer fetus DNA identification kit and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1. Pretreatment of test samples

[0060] There are two types of test samples, 1 is genuine deer fetus (fresh), and 2 is genuine deer fetus (dry), both provided and identified by Longtan Mountain Deer Industry Company in Jilin City). Take 1g for each, scrub and rinse with deionized water , dried at room temperature, and irradiated with ultraviolet light for more than 30 minutes. Use a mortar to grind the sample to about 2-3 mm, place it in a centrifuge tube, add the corresponding volume of treatment solution (mainly containing sucrose, Tris-HCl, Na 2 EDTA), shaking in a 56°C incubator for 4h. After centrifugation at 12000r / min for 10min, the supernatant was discarded, and the pellet was used for later use.

[0061] 2. Mitochondrial DNA Extraction

[0062] (1) Sample lysis Add 5ml of lysate to the pretreated sample, place it in a water bath, and shake it in a water bath at 56°C for 4 hours.

[0063] (2) Precipitation Add 2ml of precipitation solution directly to the l...

Embodiment 2

[0077] Example 2 Identification of Commercially Available Deer Fetus Medicinal Materials

[0078] 1. Materials

[0079] 2 samples of commercially available deer fetus powder, 2 genuine deer fetuses (provided and identified by Longtan Mountain Deer Industry Company in Jilin City); counterfeit sheep fetuses, pig fetuses, and cow fetuses (provided by Longtan Mountain Deer Industry Company in Jilin City), mutton , pork and beef (purchased from RT-Mart Supermarket in Jilin City).

[0080] 2. Method

[0081] 2.1 Pretreatment of test samples Take 1g of each test sample, put it in a centrifuge tube, add the corresponding volume of treatment solution (mainly containing sucrose, Tris-HCl, Na 2 EDTA), shaking in a 56°C incubator for 4h. After centrifugation at 12000r / min for 10min, the supernatant was discarded, and the pellet was used for later use.

[0082] 2.2 Mitochondrial DNA extraction

[0083] (1) Sample lysis Add 5ml of lysate to the pretreated sample, place it in a water ...

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Abstract

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a deer fetus DNA identification kit and method. The deer fetus DNA identification kit comprises a mitochondria DNA extraction system, a PCR (polymerase chain reaction) system and a result observation system. The deer fetus DNA identification method includes the steps of detection sample pretreatment, mitochondria DNA extraction, PCR primer designing and synthesis, building of the PCR system and result judgment, wherein judgment standards includes that deer fetuses are judged to be qualified if 563bp strips appear and judged to be pseudo if only multiple strips or no strips appear. The PCR identification method has the advantages of simplicity, quickness and reliable detection result, and specificity of qualified and pseudo deer fetuses can be accurately differentiated and identified at the same time.

Description

technical field [0001] The invention relates to a DNA identification kit and an identification method for identification of deer fetus and its easily mixed species by using biotechnology. Background technique [0002] Deer fetus is the fetus and placenta of deer family animals. Its original animal is deer. Deer is the general name of deer family animals and belongs to mammalian Artiodactyla. At present, there are 16 genera and about 52 species in the world, and there are 9 genera and 16 species in my country. Deer are sika deer (CervusnipponTemminck), sambar (CervusunicolorKerr), white-lipped deer (C.albirostrisPrzewalski), red deer (C.elaphusL.) and so on. The sika deer, red deer, white-lipped deer, and sambar deer with antler value are called antler deer. Antler deer are both economic animals and medicinal animals, so the economic value of deer is very high. The sika deer is a national first-class protected animal, and the sika deer is the main species of deer raised art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q1/686C12Q2600/16C12Q2565/125C12Q2545/113
Inventor 艾金霞李明成王雪松张丽华王冰梅高丽君李萧涵李梓僮
Owner BEIHUA UNIV
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