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Method for fast reproducing pinellia ternata

A fast technology of Pinellia ternata, applied in the field of seed breeding and expansion of the biological medicinal material Pinellia ternata, can solve the problems of increased workload, repeated pollution, bulb carrying bacteria, etc., and achieve the effects of shortening the regeneration cycle, increasing the regeneration efficiency, and reducing the pollution rate.

Inactive Publication Date: 2016-01-06
韦丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively convenient to use bulbs as explants for regeneration, but due to the serious bacterial contamination of bulbs, it often leads to incomplete disinfection, repeated pollution during the cultivation process is very serious, increasing the workload, and the regeneration efficiency of scales as explants is low. Not very ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for rapid propagation of Pinellia ternata, including the following steps:

[0023] 1) Select healthy Pinellia plants without scars, and cut the petioles for later use;

[0024] 2) Put the petiole in step 1) into 70% soak for 40S, take it out and rinse it with clean water;

[0025] 3) Soak the petiole obtained in step 2) in a 5% potassium permanganate solution for 0.5 min, and then rinse it with water;

[0026] 4) Put the petiole body obtained in step 3) into MS solid medium for inducing growth of adventitious buds to obtain tissue blocks with adventitious bud clusters;

[0027] 5) The tissue block obtained in step 4) is made into a longitudinally divided tissue block, and the bud elongation culture is performed on the bud elongation medium;

[0028] 6) When the adventitious shoots obtained in step 4) are elongated to 5 cm, they are cut and placed in a rooting medium to induce rooting culture to obtain pinellia seedlings.

[0029] The MS solid medium is MS+BA1.8mg / L+NAA0.8m...

Embodiment 2

[0036] A method for rapid propagation of Pinellia ternata, including the following steps:

[0037] 1) Select healthy Pinellia plants without scars, and cut the petioles for later use;

[0038] 2) Put the petiole in step 1) into 80% soak for 45S, and then rinse it off with water;

[0039] 3) Soak the petiole obtained in step 2) in a 6% potassium permanganate solution for 0.8 min, and then rinse it with water;

[0040] 4) Put the petiole body obtained in step 3) into MS solid medium for inducing growth of adventitious buds to obtain tissue blocks with adventitious bud clusters;

[0041] 5) The tissue block obtained in step 4) is made into a longitudinally divided tissue block, and the bud elongation culture is performed on the bud elongation medium;

[0042] 6) When the adventitious shoots obtained in step 4) are elongated to 7 cm, they are cut and placed in a rooting medium to induce rooting culture to obtain pinellia seedlings.

[0043] The MS solid medium is MS+BA2.2mg / L+NAA1.2mg / L, pH5....

Embodiment 3

[0050] A method for rapid propagation of Pinellia ternata, including the following steps:

[0051] 1) Select healthy Pinellia plants without scars, and cut the petioles for later use;

[0052] 2) Put the petiole in step 1) soaked in 90% for 50S, and rinse with water after taking it out;

[0053] 3) Soak the petiole obtained in step 2) in a 7% potassium permanganate solution for 1 min, and then rinse it off with water;

[0054] 4) Put the petiole body obtained in step 3) into MS solid medium for inducing growth of adventitious buds to obtain tissue blocks with adventitious bud clusters;

[0055] 5) The tissue block obtained in step 4) is made into a longitudinally divided tissue block, and the bud elongation culture is performed on the bud elongation medium;

[0056] 6) When the adventitious shoots obtained in step 4) are elongated to 8 cm, they are cut and placed in a rooting medium to induce rooting culture to obtain pinellia seedlings.

[0057] The MS solid medium is MS+BA2.0mg / L+NAA1.0...

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Abstract

The invention relates to a method for fast reproducing pinellia ternate. The method comprises the following steps that a pinellia ternate seedball is taken, inducing growing culturing is carried out on an adventitious bud, a tissue block with adventitious bud clusters is obtained, and root induction culturing is carried out to obtain a pinellia ternate seedling. The method is high in regeneration efficiency, the bud ratio is about 100%, the pollution rate is lowered, repeated pollution is avoided in the culturing process, and inductivity can be improved and the regeneration period can be shortened by adjusting culture medium conditions.

Description

Technical field [0001] The invention relates to a method for breeding and expanding biological medicinal materials Pinellia ternata seeds. The technology is directly applied to the breeding and expanding of biological medicinal materials, and in particular to a rapid propagation method of Pinellia ternata. Background technique [0002] Pinellia (scientific name: Pinelliaternata), also known as Di Wen, Morita, etc., belongs to the order Araceae. It is widely distributed in China's Yangtze River Basin, Northeast China and North China. Medicinal plants have the functions of drying dampness and reducing phlegm, reducing adverse effects and relieving vomiting, and relieving boils and swelling. It is also distributed in Tibet, with an altitude of about 3000m. [0003] Pinellia scale is the most commonly used tissue culture material for rapid propagation. Studies have shown that the leaves and ovary of Pinellia can also regenerate to form seedlings. Although it is relatively convenien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦丽
Owner 韦丽
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