High performance liquid chromatography detection method of compound nightshade inflammation-diminishing tablets

A technology of compound nightshade anti-inflammatory tablets and high-performance liquid chromatography, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems such as the relatively difficult detection method of the active ingredients of compound nightshade anti-inflammatory tablets, and achieve methanol elution capacity Improve and adjust mobile phase polarity and reduce tailing effects

Inactive Publication Date: 2015-12-30
PHARMA FACTORY GUANGXI MEDICAL UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] Compound Solanum Solanum Anti-inflammatory Tablets is a newly developed drug, and the raw materials of Compound Solanum Solanum Anti-inflammatory Tablets contain 3 traditional Chinese medicines and there are complex interactions among the ingredients of the traditional Chinese medicines, leading to the establishment of a comprehensive and effective reflection of the internal quality and quality of Compound Solanum Solanum Anti-inflammatory Tablets There are great difficulties in the detection method of the effective components of the curative effect. Therefore, there is a demand in the field for a detection method that can fully characterize the compound Solanum nigrum Anti-inflammatory Tablets.

Method used

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Embodiment 1

[0049] The contents of gallic acid, quercetin, kaempferol, solanine and solanine in compound Solanum nigrum tablets were detected by high performance liquid chromatography (HPLC) with isocratic elution.

[0050] 1. The content of gallic acid, quercetin, and kaempferol in Compound Solanum nigrum Anti-inflammatory Tablets is detected by high-performance liquid chromatography detection method comprising the following steps:

[0051] 1) Chromatographic conditions:

[0052] Chromatographic column: Octadecylsilane bonded silica gel is used as filler;

[0053] Mobile phase: a mixture of methanol and phosphoric acid solution, the volume fraction of phosphoric acid solution is 0.4%, and the volume ratio of methanol and phosphoric acid water is 55:45;

[0054] Flow rate: 1mL / min;

[0055] Column temperature: 25°C;

[0056] Gradient wavelength detection: 0~15min, the detection wavelength is 270nm; 15~30min, the detection wavelength is 362nm;

[0057] 2) Prepare a mixed control soluti...

Embodiment 2

[0073] The contents of gallic acid, quercetin, kaempferol, solanine and solanine in compound Solanum nigrum tablets were detected by gradient elution by high performance liquid chromatography (HPLC).

[0074] 1. The content of gallic acid, quercetin, and kaempferol in Compound Solanum nigrum Anti-inflammatory Tablets is detected by high-performance liquid chromatography detection method comprising the following steps:

[0075] 1) Chromatographic conditions:

[0076] Chromatographic column: Octadecylsilane bonded silica gel is used as filler;

[0077] Mobile phase: Mobile phase A is methanol, mobile phase B is 0.4% phosphoric acid solution, and gradient elution is carried out. The gradient elution procedure is as follows, wherein the mobile phase ratios are all volume percentages:

[0078] 0~13min, mobile phase A: 2%, mobile phase B: 88%;

[0079] 13-14min, mobile phase A: 2%-52%, mobile phase B: 48%-88%;

[0080] 14-30min, mobile phase A: 52%, mobile phase B: 48%;

[0081]...

Embodiment 3

[0105] The contents of gallic acid, quercetin, kaempferol, solanine and solanine in compound Solanum nigrum tablets were determined by gradient elution by ultra-high performance liquid chromatography (UPLC).

[0106] The ultra-high performance liquid chromatography principle in this test is basically the same as the high performance liquid chromatography in embodiment 2, and the changed place has the following points:

[0107] (1) Use small particles and high-performance particulate stationary phases. HPLC chromatographic columns, such as: common octadecyl silica gel bonded columns, have a particle size of 5um, while UPLC chromatographic columns can reach 3.5um, or even 1.7um, so that the pore size is more conducive to material separation.

[0108] (2) Use of high-pressure infusion pumps. Due to the reduced particle size of the chromatographic column used, the pressure generated during use is naturally multiplied. Therefore, the infusion pump of the liquid chromatography is ...

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Abstract

The invention discloses a high performance liquid chromatography detection method of compound nightshade inflammation-diminishing tablets. The compound nightshade inflammation-diminishing tablets are mainly prepared from nightshades, hydrocotyle sibthorpioides and pithecellobium clypearia; the detection method is used for mainly detecting the content of gallic acid, quercetin, kaempferol, solasonine and solamargine in the compound nightshade inflammation-diminishing tablets. Phenolic acids substances including the gallic acid, the quercetin, the kaempferol and the like, and alkaloids substances including the solasonine and the solamargine in the inflammation-diminishing tablets can be detected by steps; the detection method has high detection accuracy and rapid detection speed, and can be used for determining the content of the inflammation-diminishing tablets; the method guarantees the accuracy and advancement of quality detection standards of the inflammation-diminishing tablets are guaranteed, and can be used as important indexes of quality control of the inflammation-diminishing tablets.

Description

technical field [0001] The invention relates to the field of drug detection. More specifically, the present invention relates to a high-performance liquid chromatography detection method of compound Solanum nigrum anti-inflammatory tablets. Background technique [0002] Compound Chinese medicine is the main form of TCM clinical medicine, and it is the characteristic and essence of TCM. Traditional Chinese medicine is a complex system composed of multiple components and factors. The diversity and complexity of its chemical components are the material basis for its curative effect. Establish a modern quality control system in line with the characteristics of traditional Chinese medicine, overcome the problems of quality analysis and evaluation of traditional Chinese medicine, and improve the current status of traditional Chinese medicine. Quality control methods have become the subject of active research. [0003] The establishment of a quality control system for traditional...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 王捷蒋伟哲叶勇苏志恒蒋敏捷黄增琼
Owner PHARMA FACTORY GUANGXI MEDICAL UNIV
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